300kV Cryo-TEM

本提案旨在建立一个高端低温电子显微镜（cryo-EM）设施，该设施配备了当前主要低温电子显微镜技术的仪器，即单粒子分析，低温电子断层扫描（cryo-ET）和电子衍射，作为大学的核心设施。这将使弗莱堡大学在当前结构和细胞生物学的这些开创性技术方面具有竞争力。为了实现这一目标，需要配备快速计数检测器和能量滤波器的高端300千伏透射电子显微镜。为了在冷冻et中制备样品并进一步分析，还需要冷冻聚焦离子束扫描电子显微镜与相关的光学和电子显微镜装置相结合。最后，由于这些仪器产生异常大量的数据，用于预处理和数据分析的专用服务器和存储阵列是必不可少的。

This proposal aims to set-up a high-end cryo-electron microscopy (cryo-EM) facility that is equipped with instrumentation for the major current techniques of cryo-EM, namely single-particle analysis, cryo-electron tomography (cryo-ET) and electron diffraction as a university core facility. This will allow the University of Freiburg to be competitive in these seminal tech-niques of current structural and cellular biology. To realize this, a high-end 300 kV transmis-sion electron microscope with a fast-counting detector and an energy filter is necessary. For sample preparation in cryo-ET and further analysis, a cryo-focussed ion beam - scanning electron microscope in combination with a correlative light-and electron microscopy set-up is also required. Finally, as these instruments produce exceptionally large amounts of data, a dedicated server and storage arry for preprocessing and data analysis are essential.