Cloning of avirulence genes in Pyricularia spp. using super SAGE and comparative analysis of their instability

我们发现，AVR-Pita，AVR-Pia，AVR-Pii，AVR-Pik，稻瘟病菌无毒基因，在其基因组位置是高度可变的，发生在染色体1至7和额外染色体。它们的两侧是逆转录转座子，这可能有助于它们在进化过程中响应相应的抗性基因在基因组中的“多重易位”。我们还发现了一个无毒基因，没有经历多次易位。我们认为无毒基因的稳定性是可以预测的基础上，其染色体位置

We found that AVR-Pita, AVR-Pia, AVR-Pii, AVR-Pik, avirulence genes of the rice blast fungus, are highly variable in its genome location, occurring in chromosomes 1 through 7 and supernumerary chromosomes. They were flanked by retrotransposons, which presumably contributed to their "multiple translocation" across the genome during the course of evolution in response to corresponding resistance genes. We also found an avirulence gene that has not undergone multiple translocation. We suggest that the stability of avirulence genes is predictable on the basis of the their chromosomal locations