Modified enzyme which target to neuronal cells to cross blood brain barrier

以神经元为靶点的修饰酶通过血脑屏障的研究我们尝试用神经氨酸酶和半乳糖苷酶修饰胎盘葡萄糖脑苷脂酶，并将其静脉注射到小鼠体内。本研究追踪了小鼠脑、肝、肾等组织中葡萄糖脑苷脂酶的活性。注射15分钟后酶活性达到最大值，脑内可见明显的酶活性染色。这些数据表明，mamose终止酶可用于治疗神经元病性戈谢病。卵磷脂-胆固醇-氨基苯基糖苷（7：2：1）脂质体能有效地穿过血脑屏障进入小鼠脑内.此外，腹腔注射的脂质体被排他地分布在富含溶酶体的部分中并被胶质细胞摄取。提示血脑屏障细胞和神经胶质细胞对甘露糖有识别作用，可用于治疗溶酶体贮积病引起的脑损伤。

MODIFIED ENZYME WHICH TARGFRS TO NEURONAL CELLS TO CROSS BLOOD BRAIN BARRIERWe tried to prepare modified placental glucocerebrosidase treated with neuraminidase and galactosidase and mumose terminal glucocerebrosidase was injected intravenously into mouse. We chased enzyme activities of glucocerebrosidase in mouse brain, Ever, kidney and other tissues. The maximum activity of the enzyme was obtkned after 15 minute injection, and significant staining of-enzyme-activity was found in the brain. These data suggest that mamose terminated enyzme could be useful for the treatment of neuronopathic Gaucher disease. Liposomes prepared from lecithincholesterol- aminophenylmwmoside (7 : 2 : 1) were efficiently incorporated into mouse brain across the blood brain barrier. Furthermore, hposomes injected intraperitoneauy were exclussively distributed into lysosome rich fraction and taken up by glial cells. These data suggest that blood brain barrier cells and glial cells recognize mannose and can be used for the treattnent of brain damage by lysosomat storage disease.