Analysis of the mechanism of eukaryotic transcription initiation and its regulation based on the TATA box-binding factor TFIID

TATA盒结合因子TFIID在转录激活机制中起重要作用。我们通过多种方法阐明了TFIID的功能和结构在真核转录启动中的意义及其调控。果蝇TAF_&lt；II&gt；230与转录激活剂VP162竞争结合的转录激活机制TFIID亚基之一的人类TAF_&lt；II&gt；100特异性相互作用的功能分析3。组蛋白折叠基序参与人TAF；&lt；II&gt；80与TAF_&lt；II&gt；224相互作用的分析转录延伸因子S-II家族成员在睾丸生殖细胞减数分裂中和减数分裂后限制性表达的分析含有WD40结构基序的裂殖酵母72 kDa TFIID亚基三方结构的分子遗传学研究HIV-1-Tat相互作用蛋白Tip607组蛋白乙酰转移酶活性的新底物特异性的阐明。TFIID亚基WD40重复基序突变导致分裂酵母胞质分裂缺陷的阐明。组蛋白乙酰转移酶如何选择核心组蛋白中赖氨酸残基的机制假说9。GBF的分离和初步鉴定：一种新型的DNA结合锌指蛋白，能与HIV-1启动子10富含GC的结合部位结合。体外核心组蛋白Tip 60对6种特定类型赖氨酸乙酰化的分析。通过分离与酿酒酵母SSL1相应的基因，对TFIIH亚基进行了分析，发现存在保守的结构基序

TATA box-binding factor TFIID plays a crucial role in machanisms of transcription activation. We elucidated the significance of function and structure of TFIID in eukaryotic transcription initiation and its regulation by several methods.1. Mechanism of the transcriptional activation through the competitive binding of Drosophila TAF_<II>230 and the transcriptional activator VP162. Functional analysis of specific interactions of human TAF_<II>100, one of the TFIID subunit3. Analysis of the involvement of the histone fold motifs in the mutual interaction between human TAF_<II>80 and TAF_<II>224. Analysis of restricted expression of a member of the transcription elongation factor S-II family in testicular germ cells during and after meiosis5. Molecular genetic elucidation of the tripartite structure of the Schizosaccharomyces pombe 72 kDa TFIID subunit which contains a WD40 structural motif6. Elucidation of novel substrate specificity of the histone acetyltransferase activity of HIV-1-Tat interactive protein Tip607. Elucidation of defect in cytokinesis of fission yeast induced by mutation in the WD40 repeat motif of a TFIID subunit8. Hypothesis of mechanism of how histone acetyltransferases select lysine residues in core histones9. Isolation and initial characterization of GBF ; a novel DNA-binding zinc finger protein that binds to the GC-rich binding sites of the HIV-1 promoter10. Analysis of acetylation of six lysines of a specific class by Tip 60 in core histones in vitro11. Analysis of TFIIH subunit, through isolation of the gene from Schizosaccharomyces pombe corresponding to that of Saccharomyces cerevisiae SSL1, reveals the presence of conserved structural motifs