A study on exocrine substances of Trichinella that induce dedifferentiation and transformation of infected muscle cell

基于这两个原因，利用旋毛虫的cDNA文库实现了多肽的大批量、高纯度生产。一种是获得抗原，用于免疫诊断和/或免疫反应分析。另一种是获得和分析可能改变宿主肌肉细胞基因表达的蛋白质，即所谓的护理细胞形成。我们构建了旋毛虫和假旋毛虫的cDNA文库，并获得了一些可能重要的多肽。该方法包括肌幼虫mRNA的提取、cDNA文库的构建、大肠杆菌菌落抗感染血清的免疫筛选。获得10个阳性克隆，其中5个进行了测序。同源性分析表明，克隆111与蛋白水解酶抑制剂有同源性，克隆132与Despommier报道的同源性较高。其他克隆是独一无二的，与之前报道的序列没有相似性。免疫印迹分析合成肽与抗ES抗体或感染血清反应，或不反应。

Mass and pure production of peptide using cDNA library of Trichinella was achieved for the two reasons. One is to obtain antigen for immunodiagnostic purposes and/or immunoresponse analysis. The other is to obtain and analysis protein that may alter gene expression of host muscle cells, known as nurse cell formation. We have constructed cDNA library of T spiralis and T pseudospiralis, and produced some of possibly important peptides. The method includes isolation of mRNA from muscle larvae, cDNA library construction, immunoscreening of E coli colony against infected sera. Ten-positive clones were obtained, 5 out of which were sequenced. Homology analysis showed Clone 111 has homology to protenase inhibitor, and Clone 132 seemed to be identical to one reported by Despommier. Other clones were unique showing no similarity to previously reported sequences. Western blot analysis of synthesized peptides reacted against anti ES antibody or infected sera, or failed to react.