A study on factors promoting the development of cancer in the remnant stomach.

对促进残胃发育的因素进行了研究，得到以下结果：建立了大鼠腺胃黏膜鸟氨酸脱羧酶（ODC）活性测定体系。研究表明胆汁酸可诱导大鼠腺胃ODC活性。1)将na -牛磺胆酸钠注入大鼠胃内，诱导胃腺粘膜ODC活性。注射后4 ~ 6 h检测活性，1.75 g/Kg体重时活性最高。2) na -牛磺酸去氧胆酸盐和na -牛磺酸去氧胆酸盐诱导ODC活性相似。3)胃窦的活性最高，腺胃体次之，前胃次之。结果表明，致癌物后给药胆汁酸可促进大鼠残胃肿瘤的诱导。1)将大鼠分为4组。第1组给予MNNG(83<micrn>g/ml) 15周，第16周采用Roux eny法进行胃切除术，以防止十二指肠反流；第2组在给予MNNG和胃切除术后给予含有0.25% na -牛胆酸盐的饲粮。3组胃切除术后饲喂含胆汁酸饲粮，不给MNNG， IV组只给MNNG。2)在残胃对应部位，I组肿瘤发生率为6/22(27.3%)，2组为9/22(40.8%)，3组为0/22 (0%)，IV组为2/24(8.3%)，2组发生率显著高于IV组（p<0.05）。因此，胆汁酸的使用促进了残胃肿瘤的发展。提示胆汁酸是促进残胃发育的重要因素。

Factors promoting the development of the remnant stomach were investigated and the following results were obtained.1. The assay system to measure ornithine decarboxylase (ODC) activity in the glandular stomach mucosa of rats was established.2. It was elucidated that bile acids induced ODC activity in the glandular stomach of rats.1) Na-taurocholate was instillated into the stomach of rats and ODC activity was induced in the glandular stomach mucosa. The activity was detected 4 to 6 hours after the instilation and the maximum one was obtained at the dose of 1.75 g/Kg body weight.2) Na-taurodeoxycholate and Na-taurochenodeoxycholate similarly induced ODC activity.3) The activity was highest in the antrum, followed by the body of the glandular stomach and the forestomach.3. It was elucidated that the administration of bile acids after the carcinogen promoted the induction of tumors in the remnant stomach of rats.1) Rats were devided into 4 groups. MNNG(83<micrn>g/ml) was administered for 15 weeks and gastrectomy was performed by the Roux en Y method which would prevent duodenal reflux at the 16th weeks in group I while the diets containing 0.25% Na-taurocholate were gilven following the administration of MNNG and gastrectomy in group 2. Bile acid-containing diets was given after gastrectomy without MNNG administration in group 3 and only MNNG was administered in group IV.2) The incidence of tumors was 6/22 (27.3%) in group I, 9/22 (40.8%) in group 2 , 0/22 (0%) in group 3 and 2/24 (8.3%) at the site corresponded to the remnant stomach in group IV. The incidence of group 2 was significantly higher than that of group IV (p<0.05). Thus, the administration of the bile acid promoted the development of tumor in the remnant stomach.These results suggested that bile acids are factors promoting the development of the remnant stomach.