Detection and quantitation of rice dwarf virus-related nucleic acid in infected cells.

水稻矮缩病毒属于植物呼肠孤病毒亚组1，基因组由12个dsRNA片段组成。将9号和10号基因片段经均聚后克隆到pBR 322的Pst1位点。片段9和片段10均获得了表观长度约为1300-1400个核苷酸的插入片段。片段10的dscDNA也是用Gublar和Hoffman(1983)的方法从体外合成的病毒转录本中获得的。其中一个长约1,000个核苷酸的克隆，通过对转录本和克隆的引物延伸和双脱氧测序分析，发现该克隆含有完整的转录本5‘端。测定了基因片段10的完整核苷酸序列。

Rice dwarf virus (RDV) belongs to plant reovirus subgroup 1 and its genome consists of 12 segments of dsRNA. Gene segments 9 and 10 were cloned into Pst1 site of E. coli plasmid pBR 322 after homopolymeric tailing. cDNA inserts with apparent lengths of about 1300-1400 nucleotides were obtained for both segments 9 and 10. dscDNA of segment 10 was also made from viral transcript synthesized in vitro by a method of Gublar and Hoffman (1983). One clone of about 1,000 nucleotides in length was found to contain complete 5'end of the transcripts by primer extension and dideoxy sequencing analyses of both transcripts and the cDNA clone. Complete nucleotide sequence of gene segment 10 was determined.