Regulation of glucose metabolism in chicken kidney

研究了动脉系统和门静脉系统灌注的鸡肾对葡萄糖代谢的调节。在两种灌注系统中，以5 ml/min / g肾脏的流速灌注含氧缓冲溶液，肾脏实现氧合。丙酮酸，一种氧化底物，在灌注的鸡肾中被发现是比乳酸更好的糖异生前体。喹啉酸盐是胞质磷酸烯醇丙酮酸羧激酶的抑制剂，对乳酸盐和丙酮酸盐的糖异生有抑制作用，但对琥珀酸盐、苹果酸盐和< α > -酮戊二酸盐没有抑制作用。鸡肾糖异生发生在皮质，糖酵解发生在髓质结构。此外，通过<Na^+> -依赖系统运输的琥珀酸和通过<Na^+> -不依赖系统通过肾基底外侧运输的单羧酸是糖异生的良好底物。

The regulation of glucose metabolism was studied in chicken kidney perfused with either an arterial system or a portal system.A. Provided that kidneys were perfused with oxygenated buffer solution at a flow rate of 5 ml/min per g of kidney in both perfusion system, oxygenation of the kidney was achieved.B. Pyruvate, an oxidized substrate, found to be a much better gluconeogenic precursor than lactate in the perfused chicken kidney.C. Quinolinate, an inhibitor of cytosolic phosphoenolpyruvate carboxykinase, inhibited gluconeogenesis from lactate and pyruvate but not from succinate, malate and <alpha> -ketoglutarate.D. Chicken renal gluconeogenesis occurs in the cortex and glycolysis occurs in medullary structures. Furthermore, succinate, transported by a <Na^+> -dependent system and monocarboxylic acids transported by a <Na^+> -independent system through the basolateral side of the kidney are good substrates for gluconeogenesis.