Study of improved method for detecting mutagenic activities of volatile and gaseous compounds.

由于气体或挥发性化合物的致突变性尚未确定，我们开发了一种简单的冒泡法来检测这些化合物的致突变性活性。以氯乙烯和环氧乙烷为例，用这种简单的方法进行了测试。将氯乙烯（VC）（100%）在试管中泡10-15分钟，吸收到二甲基亚砜（DMSO）中。然后将上述DMSO 0.1ml，新鲜培养的试验菌株（E.coli WP2uvrA/pKM） 0.1ml和S9Mix 0.5ml混合在气密封小瓶中，在37℃下摇摇30-120分钟，并将2ml上琼脂倒在最小葡萄糖琼脂板上。在37℃孵育48小时后，对Trp反射物进行评分。另一方面，用气相色谱法测定上述DMSO中VC的浓度。用该方法进行了环氧乙烷（EO）（12%）的致突变性试验。将EO吸收到10ml乙醇中。用不含S9Mix和预孵育的鼠伤寒沙门氏菌TA100进行EO诱变试验。用气相色谱法对EO进行定量分析。VC和EO均呈阳性，且呈剂量效应。VC和EO的回归曲线分别为Y=0.2X + 205.3和Y=0.157X + 157.3 （p < 0.001）。这种冒泡法对于定量检测气体化合物中的诱变活性是有用和简单的。

As the mutagenicity for gaseous or volatile compounds has not been established, we have developed a simple method by bubbling for detecting the mutagenic activities in these compounds.Vinyl chloride and ethylene oxide, for example, were tested by this simple method.Vinyl chloride(VC)(100%) was absorbed into dimethylsulfoxide(DMSO) in a tube by bubbling for 10-15 minutes. Then 0.1ml of the above DMSO, 0.1ml of a fresh cultured tester strain (E.coli WP2uvrA/pKM) and 0.5ml of S9Mix were mixed in a gas tight vial, shaked at 37 C for 30-120 minutes and poured with 2ml of top agar onto a minimal glucose agar plate. Trp revertants were scored after incubation at 37 C for 48 hours. On the other hand, the concentration of VC in the above DMSO was measured by gaschromatography.The mutagenicity test of ethylene oxide(EO)(12%) was performed by this method. EO was absorbed into 10ml of ethylalcohol. The mutagenicity test for EO was carried out by the same method using S.typhimurium TA100 without S9Mix and preincubation. The quantitative analysis of EO was performed by gaschromatography.The positive results with dose-response effects were observed both VC and EO. The regression curves are Y=0.2X + 205.3 in VC and Y=0.157X + 157.3 in EO (p 0.001).This bubbling method is useful and simple for detecting mutagenic activities in gaseous compounds, quantitatively.