In vivo study of radioimmunodetection and radioimmunotherapy with I-131 labeled monoclonal antimelanoma antibody

(1)放射性同位素标记的单克隆抗体有望用于播散性恶性肿瘤的显像和治疗。(2)本文研究了抗黑色素瘤抗体（AMAB）在小鼠黑色素瘤中的不同分布。(3)将BALB/C骨髓瘤细胞与同系B16黑色素瘤细胞致敏的C57 BL/6脾细胞融合，获得单克隆抗体。(4)初步研究结果表明，放射性同位素标记的单克隆抗体在荷瘤小鼠体内特异性蓄积，并且在宏观放射自显影上，这些抗体在肿瘤内分布不均匀。(5)将约100 <mu>Ci的I-131标记抗体（M2590）和30 <mu>Ci的I-125标记抗体（M562）同时注射到携带同基因小鼠黑色素瘤的小鼠中。(6)在制备小鼠标本后一周内，在胶片上获得I-131标记抗体的分布。约3个月后，用双核素放射自显影技术观察小鼠抗体的不同分布。(7)这一结果表明，两种不同的抗体在肿瘤中的优先选择是由于针对肿瘤表面上的决定簇的抗体，并且多克隆抗体可以被给予更高的肿瘤摄取，并且可用于放射免疫检测和放射免疫治疗。

(1) Radioisotope labeled monoclonal andibodies are going to use for imaging and treatment of disseminated malignant tumors.(2) This study was undertaken in the differrent distributions of anti-melanoma antibodis(AMAB) in the mouse melanoma.(3) The moenoclonal antibodies were obtained by fusion of BALB/C myeloma and C57BL/6 spleen cells primed with syngenic B16 melanoma cells.(4) The initial findings show that radioisotope labeled monoclonal antibody is accumulated in melanoma bearing mice specifically and that these anti-bodies are distributed inhomogeneously in the tumors on macro-autoradiogram.(5) About 100 <mu> Ci of I-131 labeled antibody(M2590) and 30 <mu> Ci of I-125 labeled antibody(M562) were injected into the mice bearing syngenic mouse melanoma at the same time.(6) The distribution of I-131 labeled antibody was obtained on film within one week after the preparation of mouse specimen. About three months later the different distributions of the mouse antibody were observed on autoradiogram with the double nuclide technique.(7) This result suggests that preferential accumlations of two different antibody in the tumors were due to the antibodies directed against a determinant on tumor surface and that the polymonoclonal antibodies can be given to higher tumor uptakes and be useful for radioimmunodetection and radioimmunotherapy.