Structure and function of cell membrane associated with multidirectional differentiation of lymphatic leukemia

许多报道表明，质膜糖蛋白可能作为受体与多种细胞识别有关。我们对人类和大鼠白血病细胞系不同阶段细胞表面糖蛋白的研究表明，表面糖蛋白的结构信息和天冬酰胺连接的糖链模式对了解其生物学作用至关重要，可能有助于白血病的分类。培养的白血病细胞系每次均可获得均匀的细胞群，可用于细胞表面的精确分析。KH-3A、3B和KH-4分别来源于11岁女孩和14岁男孩急性淋巴细胞白血病（ALL）患者。琼脂糖分离的5个KH-3A亚克隆经TPA诱导具有T细胞或B细胞特征。虽然KH-4具有常见ALL的特征，但KH-3A亚克隆可能起源于更不成熟的阶段。采用sds -聚丙烯酰胺凝胶电泳和肼解法研究了ATL细胞株KH-2的细胞表面糖蛋白和阿萨普赖氨酸链结构。进一步分析KH-3a可能是了解细胞分化过程中表面变化的必要条件

Many reports indicate that plasma membrane glycoproteins may be associated with various kinds of cellular recgnition as receptors. Our previous studies on cell surface glycoproteins of different stage of human and rat leukemia cell lines indicated that the structural information on surface glycoproteins and asparagine-linked sugar chain patterns was essential to understand the biological role of them, and might be useful for the classification of leukemia. For the precise analysis of cell surface, the cultured leukemia cell lines might be useful because the homogeneous cell population could be obtained everytime. KH-3A,3B and KH-4 were established from 11-year-old girl and 14-year-old boy with acute lymphoblastic leukemia(ALL). Five subclones isolated from KH-3A by agarose could be induced to have T or B cell characteristics by TPA. Although KH-4 has the characteristics of common ALL, KH-3A subclones might be originated from more immature stages.The cell surface glycoproteins and asapragine-linked sugar chain patterns of ATL cell line(KH-2)were studied by using SDS-polyacrylamide gel electrophoresis and hydrazinolysis. Further analysis of KH-3a may be necessary to know the surface changes of cell differentiation