Physico-chemical stability of blood group-specific immunoglobulins in the state of immunocomplex

免疫凝集素或沉淀素因加热或某些化学物质而失去与相应抗原的反应性。用人Ig M抗A或兔Ig G抗M血清分别致敏Se组和M组血染。用生理盐水清洗后，用福尔马林或2-巯基乙醇(2-ME)或不同浓度的溶液加热或处理。用混合凝集或放散试验检测免疫复合体状态抗体的免疫反应性，表明免疫复合体状态的抗体对150℃加热10min和高浓度的福尔马林和2-ME都是安全的。认为免疫球蛋白Ig M和Ig G虽然实质上是热和化学物质，但通过形成免疫复合体而获得新的热稳定性和化学稳定性

Immune agglutinin or precipitin loses its reactivity with the corresponding antigen by heating or some chemicals. A.Se group seliva or M group blood stain was sensitized with human IgM anti-A or rabbit IgG anti-M serum,respectively. After being washed with saline, the stains were heated or treated with formalin or 2-mercaptoethanol(2-ME) or various concentrations. The immunoreactivity of the antibodies in the state of immunecomplex was examined by the mixed agglutination or elution test, indicating IgM and IgG antibodies in the state of immunecomplex were proof against heating up to 150C for 10 min and against formalin and 2-ME of high concentrations. It is considered that IgM and IgG acguire a new property of heat and chemical stability by formation of immunecomplex although they are substantially heat and chemical fabile