A study of the effect of human monocytes on the mechanisms of bone resorption

Mundy等人最近被证明是一个演示。（1977年）-单核噬细胞存在于阿尔维奥拉骨骼中，它已经被摧毁或在阿尔维奥拉骨骼的重建表面上。此外， Heersche （1978年）报告说，骨弹性骨骼分解可能是由纤维状细胞和单细胞衍生细胞的作用引起的，也是如此。在另一方面，Kahn等人提出了这一建议。（1975年）和Ash等人。（1980年）宏观结构可能与骨细胞有关，因此，我们研究了在体外单细胞的骨骼分解活动和在这种活动中对宿主准备好的文化外围血液白血病（PBL-sup）的影响。使用^<45>Ca-标记的合成羟基磷灰石（^<45>Ca-HA），人类单体的骨骼修复活动被调查，以及PBL-sup对周期性疾病（PPD）患者的影响，以及对该单体活动的临床上高水平个人对测试。《骨头-矿物毁灭的机制》（《<45>Ca release》） ... More 来自^<45>Ca-HA）人类monocytes被调查，结果如下： 1。人类个体明显地受到了来自Ca-HA的<45>^ Ca版本<45>的启发，依赖于培养时间和细胞集中;而且这种活动显著地增加了PBL-sup，而不是来自健康患者的PPD。2. PBL-sup （PPD或健康患者）的补充作用导致氮蓝四氮杂素减少活动和[^3 H]单细胞的三胺增加。3. Monocytes在48小时培养中由lightmicroscopy和electronmicroscopy制成。4.通过添加PHA-sup， monocytes-adhering activity到HA是增强的、显著的和释放的数量的^<45>Ca从^<45>Ca-H a被monicytes增加。5.根据细胞色素B的补充（1克/毫升-10克/毫升），从<45>^<45>Ca-HA释放的数量减少到集中。6.从<45>^<45>Ca-HA在24小时的文化中检测到的媒体Ca，<45>但在96小时的文化中检测到的细胞内Ca。这些结果建议，单胞菌的骨骼-矿物破坏与钙酸在微环境中的集中有关。Less（低）

Recently it has been demonstrated by Mundy et al.(1977) that mononuclear phagocytes are present in alveolar bone which has been destroyed or on the resorbed surface of alveolar bone. Also, Heersche (1978)reported that osteoclastic bone resorption may occur by the action of fibroblast-like cells and monocyte-derived cells, too. On the other hand, it has been suggested by Kahn et al.(1975) and Ash et al.(1980) that macrophages are probably related ontogenetically to osteoclasts.Therefore, we examined the bone-resorbing activity of monocytes in vitro and effect of supernatants prepared from cultured peripheral blood leukocytes(PBL-sup) on this activity. Using ^<45>Ca-labeled synthetic hydroxyapatite(^<45>Ca-HA), the bone-resorbing activity of human monocytes was investigated, and the effects of PBL-sup from patients with periodontal disease(PPD) and from clinically hyalthy individuals on this monocyte activity were examined. And, the mechanism of bone-mineral destruction (^<45>Ca release … More from ^<45>Ca-HA) by human monocytes was investigated.The results were as follows: 1. Human monocytes apparently induced the release of^<45>Ca from^<45>Ca-HA, depending on culture time and cell concentration; and this activity was increased significantly more by addition of PBL-sup from PPD than from healthy patients. 2. The addition of PBL-sup (of PPD or healthy patients) led to increased nitro blue tetrazorium-reducting activity・and [^3H] Thymidine incorporation of the monocytes. 3. Monocytes adhering to HA were observed by lightmicroscopy and electronmicroscopy in 48 hr culture. 4. By addition of PHA-sup, monocytes-adhering activity to HA was enhanced significantly and the quantity of released ^<45>Ca from ^<45>Ca-H a by monicytes was increased. 5. By addition of cytochalasin B(1 g/ml-10 g/ml), the quantity of released^<45>Ca from^<45>Ca-HA was decreased dependently to the concentration. 6. Released ^<45>Ca in media from ^<45>Ca-HA was detected in 24 hr culture, but cell-intral ^<45>Ca was detected in 96 hr culture.These results suggest that bone-mineral destruction by monocytes is related to the concentration of citric acid in the micro-enviroment of the adherene site. Less