Efficient use of oocytes by the perfusion of farm animal ovary

为有效利用卵母细胞并检测卵巢功能，对山羊卵巢体外灌流技术进行了研究。研究了不同介质灌注对卵巢功能的影响，特别是对卵泡破裂和肿胀现象的影响。建立了一套山羊卵巢体外灌流系统。灌流装置由一个循环系统组成，包括三个主要部分：氧合器、滚柱泵和灌注室。通过对灌流液的pH值、葡萄糖和乳酸浓度以及卵巢的组织学和组织化学的一系列测定，证实了灌流卵巢的功能完整性。经过几个初步实验，尝试了9个使用含有Graafian卵泡的卵巢的实验。在九个实验中的一个实验中，两个Graafian卵泡的破裂是通过灌流山羊卵巢而发生的。然而，在其他实验中，没有发生Graafian卵泡的破裂。…的血流灌注卵巢多可引起Graafian卵泡和小有腔卵泡肿胀，并伴有间质水肿。在灌流系统中观察到的Graafian卵泡破裂可能是伪影，需要仔细选择灌流介质以避免误解。在6小时的灌流期间，葡萄糖消耗和乳酸产生持续发生。光镜显示，尽管在灌流的卵巢中有间质水肿形成，但在整个灌流过程中，几乎保持了正常的卵巢结构。在组织化学研究中，3β-HSD和G6PDH在卵巢灌流后卵泡的内膜和颗粒细胞中保持正常活性。电子显微镜观察表明，目前的灌流系统在维持卵巢组织方面并不令人满意。综上所述，如果可以改进灌流技术，家畜卵巢的灌流系统对于有效利用卵母细胞和研究卵巢功能是有用的。较少

A technique for in vitro perfusion of goat ovaries is studied to use oocytes efficiently and to examine ovarian function. The effect of perfusion with different media on ovarian function and, in particular, on the phenomenon of follicular rupture and swelling is examined. A system has been developed for the perfusion of the goat ovary in vitro. The perfusion apparatus consisted of a recycling system with three major portions: an oxygenator, a roller pump, and a perfusion chamber. The functional integrity of the perfused ovary is confirmed by serial determinations of the perfusate pH, glucose and lactate concentrations, and by histology and histochemistry of the ovary.After several preliminary experiments, nine experiments, where the ovary containing Graafian follicles was used, were tried. In one of nine experiments the rupture of two Graafian follicles occurred by perfusion of the goat ovary. However in the other experiments the rupture of Graafian follicles did not occur. Perfusion o … More f the ovary caused swelling of Graafian follicles and small antral follicles, accompanied by a stromal edema. Graafian follicular rupture observed in perfusion system may be artifacts and careful selection of the perfusion medium is needed to avoid misinterpretation.Glucose consumption and lactate production occurred continuously during the 6-hour perfusion period. It was shown by light microscopy that almost normal ovarian structure was maintained throughout the perfusion, although interstitial edema formation was noted in the perfused ovaries. In the histochemical study, normal activity of 3beta-HSD and G6PDH was maintained in the theca interna and granulosa cells of follicles in the perfused ovaries. In contrast, electron microscopic observations indicated that the present perfusion system is not satisfactory to maintain ovarian tissue.In conclusion, a perfusion system of the farm animal ovary is useful for the efficient use of oocytes and for the study of ovarian function if perfusion technique could be improved. Less