Specificity of monoclonal antibodies against Pneumocystis carinii and application of these antibodies to the immunodiagnosis

用感染卡氏肺囊虫的人、大鼠和裸鼠的肺匀浆或用梯度不连续离心法分离的肺匀浆免疫BALB/c小鼠。制备了多克隆和单克隆抗体，并鉴定了对这些抗体有反应的卡氏假单胞菌。采用免疫荧光抗体和免疫印迹技术分析了同源和异源宿主对卡氏假单胞菌抗体的反应。每一种多克隆抗体和单克隆抗体都对同源宿主的生物膜产生强烈的荧光。然而，它们与来自同源宿主的卡氏假单胞菌分离株几乎没有交叉反应性。来自不同寄主的生物在抗原性上存在差异。此外，通过使用^<125> i标记的二抗进行免疫印迹，在人卡氏疟原虫中看到的主要条带是80kd的抗原。在大鼠和小鼠的卡氏假单胞菌中，120 / 52kd抗原和120、70 / 58kd抗原也很突出。为了对人肺细胞病进行实际的免疫诊断，正在使用针对人分离物的单克隆抗体进行进一步的研究。

BALB/c mice were immunized with lung homogenates from human, rats and nude mice infected with Pneumocystis carinii or the organisms separated from those by the gradient discontinuous centrifugation method. Polyclonal and monoclonal antibodies were produced and the P. carinii organisms reactive for those antibodies were characterized. The reactions of antibodies to P. carinii from homologous and heterologous hosts were analyzed by immunofluorescent antibody and immunoblotting techniques. Each polyclonal and monoclonal antibodies gave strong fluorescence to the pellicle of the organisms from homologous hosts. However, they showed little cross-reactivity with P. carinii isolates from haterologous hosts. Antigenic differences were observed among the organisms from different hosts. Furthermore, by immunoblotting using ^<125>I-labeled secondary antibodies, the major band seen in human P. carinii was an antigen of 80kd. In P. carinii from rats and mouse, 120 / 52kd antigens and 120, 70 / 58kd antigens were also prominent respectively. For practical immunodiagnosis of human pneumocytosis, further studies are being conducted using the monoclonal antibodies against human isolates.