Identification of T antigen receptor recognizing HLA antigens and clarification of mechanism of genetic restriction

DR基因产物通常被认为参与了混合淋巴细胞反应的诱导。然而，对人类白细胞抗原-DQ和-DP抗原在MLR中的作用知之甚少。为了阐明T抗原受体识别人类白细胞抗原的机制，我们构建了DQ和DP小鼠L或人淋巴样细胞。这些DQ转基因细胞在MLR条件下能有效地刺激外周血淋巴细胞增殖。另一方面，DP转基因细胞没有表现出任何MLR活性，但成为DP特异性T细胞克隆(PLT活性)的良好靶点。这些结果提示DQ和DP抗原在免疫应答中的作用，为研究遗传限制和T抗原受体识别HLAII类抗原的机制提供了一个很好的系统。

DR gene products are commonly thought to be involved in the induction of the mixed lymphocyte reaction. However, very little is known about the role of HLA-DQ and -DP antigens in the MLR. In order to clarify the mechanism of HLA recognition by T antigen receptor, we constructed DQ and DP mouse L or human lymphoid transfected cells. These DQ transfectants were found to be efficient in stimulating peripheral blood lymphocyte proliferation under MLR condition. On the other hand, DP transfectants did not show any MLR activity, but become a good target to DP-specific T cell clone (PLT activity). These results suggest the role of the DQ and DP antigens in the immune response, providing a excellent system for investigating the mechanism underlying genetic restriction and T antigen receptor recognizing HLA class II antigens.