Chromosomal analysis of woodchuck hepatocellular carcinoma utilizing in situ hybridization technique

通过原位杂交技术鉴定了WH257 GE 10中WHV DNA整合的染色体位点。用^3H标记的WHC DNA被分配到G带图上，这是首次在土拨鼠染色体中测定。WHV DNA整合于6号染色体长臂33区（6q33）和8号染色体长臂31区（8q31）。WHV DNA整合的染色体位点在分析的传代期间是稳定的。这些结果表明，WHV DNA整合到WH257 GE 10的染色体可能与土拨鼠肝细胞癌的发病机制密切相关，整合的WHV DNA可能在维持癌细胞的特征方面发挥一定作用。

The chromosomal sites of WHV DNA integration were identified in WH257 GE10 by an in situ hybridization technique. ^3H-labelled WHC DNA was assigned to the G-banded diagram which was determined for the first time in the woodchuck chromosomes. WHV DNA was integrated into two sites; 33 region of the long arm of chromosome 6 (6q33) and 31 region of the long arm of chromosome 8 (8q31). Chromosomal sites of WHV DNA integration were stable during the passage periods analyzed. These findings suggest that WHV DNA integration into the chromosomes of WH257 GE10 might be closely associated with the pathogenesis of a woodchuck hepatocellular carcinoma, and that integrated WHV DNA might play some role in the maintenance of the characteristics as cancer cells.