Antigenetic analysis of human amniotic epithelium and its clinical application to tumor markers

1.抗人癌细胞系的单克隆抗体对人羊膜上皮细胞系的反应性：本实验室用10株人癌细胞系PC-1建立了22株鼠源性单克隆抗体（肺鳞状细胞癌），PC-10（肺鳞状细胞癌），PC-9（肺腺癌），HEp-2（咽鳞鼠细胞ca），TE-9（食管鳞状细胞癌），KATO-III（胃环细胞ca）、COLON CA（结肠腺癌）、HEK（胆管腺癌）、PANC-1（胰腺腺癌）和ZR 75 -1（乳腺腺癌）。用ELISA法检测，这些单克隆抗体均能与人结肠上皮细胞（FL和WISH）中的一种和/或两种细胞发生反应.人胎盘组织中肿瘤标志物的免疫组化研究：采用ABC法，对CA 125、CA 130、CA 19 -9、CA 50、KM 0 1和TAC 41在人早、足月胎盘组织中的定位进行了研究。抗人癌细胞系单克隆抗体所识别的肿瘤标志物均明确定位于羊膜上皮。因此，这些肿瘤标志物可命名为“羊膜上皮相关抗原（AEA）".母血、羊水及脐血AEA水平：对10例孕妇进行产前、产后母血、羊水及脐动、静脉配对血CA 125（RIA）、CA 130（RIA）、CA 19 -9（EIA）、CA 50（EIA）、TAC 41（RPHA）测定。羊水中这5种AEA的水平显著升高，而母体和脐带血中的水平保持在正常范围内。这些结果表明，羊膜上皮与癌之间存在明显的共同抗原性。此外，这些结果可能有助于开发新的肿瘤标志物。

1. The reactivity of monoclonal antibodies against human cancer cell lines on human amniotic epi-thelium cell lines: In our laboratories 22 murine monoclonal antibodies have been established by using 10 human cancer cell lines, namely PC-1(pulmonary squamouse cell ca),PC-10(pulmonary squamouse cell ca),PC-9(pulmonary adenoca),HEp-2(pharyngeal squamouse cell ca),TE-9(esophageal squamouse cell ca),KATO-III(gastric ring cell ca),COLON CA(colonic adenoca),HEK(choleduct ade-noca),PANC-1(pancreatic adnoca) and ZR75-1(breast adenoca). With use of an ELISA, these all mo-noclonal antibodies were found to react with either and/or both of human culture cells of amnio-tic epithelium (FL and WISH).2. Immunohistochemical investigation of human tumor markers in human placentae: By using ABC method, the localization of CA125.CA130,CA19-9,CA50,KM0 1 and TAC 41 were investigated in human early and term placentae. These all tumor markers recognized by mo-noclonal antibodies against human cancer cell lines were clearly localized in the amniotic epi-thelium. Thus, these tumor markers could be designated " amniotic epithlium-related antigens (AEA)".3. AEA levels in maternal blood, amniotic fluid and umbilical blood: The concentration of CA125(RIA),CA130(RIA),CA19-9(EIA),CA50(EIA) and TAC 41(RPHA) was measured in maternal blood(prior to and just after delivery),amniotic fluid and paired blood from umbilical artery and vein in 10 preagnant cases. The levels of these 5 AEAs were strikingly elevated in amniotic fluid,while the levels remained within normal limits in maternal and umbilical blood. These all find-ings indicate clear common antigenecity between amniotic epithelium and cancers. In addition, these results may facilitate to develop new tumor markers.