Search and Identification of Quinoproteins

以下酶已被处理以观察它们是否含有PQQ作为辅因子：胺脱氢酶，醇脱氢酶，醛脱氢酶，苯丙氨酸解氨酶，谷氨酸脱羧酶，酮戊二酸脱羧酶，脂氧合酶，色氨酸吡咯酶，胺氧化酶，醇氧化酶，对羟基苯甲酸羟化酶，二羟基苯甲酸脱羧酶，肌氨酸脱氢酶，胆碱脱氢酶，苄胺氧化酶，硝基烷烃氧化酶，腈水合酶，卤代氨基酸脱卤酶、芳香醇脱氢酶、异戊醇脱氢酶、聚乙二醇脱氢酶、苯胺分解酶、木质素分解酶、二腈水合酶、甲醇脱氢酶等。这些酶的酶源从真核细胞到原核细胞都有变化。本研究首次发现PQQ参与了产氢细菌、氰钴胺生物合成、花粉萌发等过程中的代谢途径。通过目前的合作研究，PQQ在底物代谢的各个方面的重要性已经得到强调。

The following enzymes have been treated to see whether they contain PQQ as cofactor : amine dehydrogenase, alcohol dehydrogenase, aldehyde dehydrogenase, phenylalanine ammonia-lyase, glutamate decarboxylase, ketoglutarate decarboxylase, lipoxygenase, tryptophan pyrrolase, amine oxidase, alcohol oxidase, p-hydroxybenzoate hydroxylase, dihydroxybenzoate decarboxylase, sarcosine dehydroginase, choline dehydrogenase, benzylamine oxidase, nitoralkane oxidase, nitrile hydratase, haloamino acid dehalogenase, aromatic alcohol dehydrogenase, sencondary alcohol dehydrogenase, polyethyleneglycol dehydrogenase, aniline decomposing enzyme, lignin decomposing enzyme, dinitrilehydratase, methanol dehvdrogenase, and so an. The enzyme sources for these enzymes were variable arranging from eucaryotes to procarytes. More than 50% of these enzymes have been confirmed to be quinoprotein.Metabolic pathway involving PQQ was indicated in hydrogcnobacteria, cyanocobalamin biosynthesis, germination of pollen for the first time in this study. Importance of PQQ in various aspects of substrate metabolism has been emphasized through the present co-operative research.