Study on Infection and Multiplication of Densonucleosis Virus of Silkworm, Bombyx mori L.

家蚕浓核病病毒（Densonucleosis virus of Bombyx mori，BmDNV）的研究工作从1988年开始，进行了三年，分为BmDNV-1、Ina-DNV、BmDNV-2、Yamanashi-DNV（yDNV）和Chinese-DNV（cDNV）。得到以下结果.采用Dot-IGSS法检测早期感染。病毒接种后10小时检测到病毒感染。阳性反应敏感性高，可达10 ng.在cDNV与NPV或CPV的混合感染中，cDNV与NPV的干扰程度较高.对DNV的DNA-1（6.56Kb）和DNV-2（6.07Kb）进行了限制性酶切图谱分析。两种DNA的碱基序列存在一定差异. yDNV和cDNV是密切相关的病毒。两种DNV的DNA末端区核苷酸序列基本相同。但cDNV DNA的酶切图谱与yDNV DNA的酶切图谱不同. cDNA-1（6.56Kb）的克隆 ...更多信息 s尝试。将其基因组DNA插入pUC 119质粒载体中，构建克隆.克隆了BmDNV-1（4.9Kb）基因。将具有完整长度的基因组DNA插入pUC 119质粒载体中，获得克隆。利用克隆p17238，分析了含有5048个核苷酸的全部碱基序列. SDS-PAGE电泳显示，cDNV有5个结构蛋白，分子量分别为41、43、48、51和100 Kd。明确了cDNV含有亚精胺、精胺和腐胺等多胺. DNV DNA的完整序列（5048个核苷酸）具有225个核苷酸的反向重复序列，末端153个核苷酸是回文的。回文蛋白可以折叠回自身以形成发夹结构，但是与AAV不同，形成T形构象的小的内部回文蛋白被折叠。持续感染两种昆虫细胞系;一种用直径为20 nm的等轴ssDNA病毒感染，另一种用直径为22 nm的等轴ssRNA病毒感染。桑葚的桑果虫Glyphodes pyloalis经常被非封闭病毒慢性感染，这些病毒在血清学上与BmDNV无法区分。结果表明，BmDNV起源于桑潜蝇非封闭型病毒，桑葚潜蝇是这些非封闭型病毒的共同习惯性宿主。少

Studies on densonucleosis virus of silkworm, Bombyx mori, (BmDNV) which is divided into BmDNV-1, Ina-DNV, and BmDNV-2, Yamanashi-DNV (yDNV) and Chinese-DNV (cDNV), had been carried out for three years since 1988. The following results were obtained.1. Dot-IGSS method was used in order to detect earlier infection with cDNV. The virus infection was detected ten hours after virus inoculation. The sensitivity showing positive reaction was high and 10ng.2. In the mixed infection of cDNV and NPV or CPV, higher degree of interference between cDNV and NPV was shown.3. Restriction enzyme mapping of the two DNAs, DNA-1 (6.56Kb) and DNV-2 (6.07Kb), of cDNV was tried. Some differences in base sequence between the two DNAs were found.4. yDNV and cDNV are closely related viruses. Between the DNAs of the two DNVs, the nucleotide sequence of the terminal region was almost same. However, the restriction pattern of the cDNV DNA was different from that of the yDNV DNA.5. Cloning of cDNV DNA-1 (6.56Kb) wa … More s tried. Genom DNA bearing the complete length was inserted into pUC119 plasmid vector and the making clone was succeeded.6. Cloning of BmDNV-1 (4.9Kb) was carried out. By inserting genom DNA bearing the perfect length into pUC119 plasmid vector, clone was obtained. And by using clone p17238, all base sequence bearing 5048 nucleotides was analyzed.7. cDNV had 5 structural proteins showing the molecular weight of 41, 43, 48, 51 and 100Kd, respectively, on SDS-PAGE gel. It was clarified that cDNV contained polyamine of spermidine, spermine and putrescine.8. The complete sequence of the DNV DNA (5048 nucleotides) has inverted repeats of 225 nucleotides and the terminal 153 nucleotides are palindromic. The palindromes can fold back on themselves to form a hairpin structure but, unlike AAV, the small internal palidrome which forms a T-shaped conformation was observed.9. Two insect cell lines were persistently infected ; one with isometric ssDNA virus 20nm in diameter and the other with an isometric ssRNA virus 22nm in diameter.10. Iarvae of the mulberry pylarid, Glyphodes pyloalis, which infest mulberry plantations, are frequently infected in a chronic state with nonoccluded viruses that are serologically indistinguishable from the BmDNVs. The results suggest that the BmDNV originated from the lyphodes nonoccluded viruses, and epizootiologically, the mulberry pyralid is a common habitual host of these nonoccluded viruses. Less