Development of "Hybrid Type" Artificial Articular Cartilage

1)将培养的胶原凝胶包埋的关节软骨细胞植入大鼠膝关节骨软骨缺损。术后4、12、24周处死大鼠，对其膝关节进行组织学检查。2)为了明确上述实验中存在的免疫学问题，我们比较了同种异体移植组和同种异体移植组的结果。结果，两组之间直到术后4周才有太大差异。术后12周，同种异体移植组修复效果明显优于同种异体移植组，尤其是软骨下骨的重组效果明显优于同种异体移植组。3)观察纤维连接蛋白对培养的鸡胚软骨细胞分化的影响。结果，通过在培养基中加入纤维连接蛋白，软骨细胞改变了它们的形状，从球形变为纺锤形。即软骨细胞去分化。4)免疫化学检测了鸡软骨细胞表面是否存在纤维连接蛋白受体。因此，我们可以验证它们识别纤维连接蛋白的RGDS序列并具有受体。5)进一步明确不同培养条件对软骨细胞分化和表型维持的影响，以修复受损关节表面。

1) We implanted the cultured articular chondrocytes embedded in collagen gel into the osteochondral defects in rat knee joints. 4, 12, 24 weeks postoperatively, the rats were sacrificed, and their knee joints were histologically examined.2) In order to make clear immunological problems in the-above-mentioned experiments, we compared the results between isografts group and allograft group. As a result, there were not so much difference between two groups until 4 weeks postoperatively. At 12 weeks after operation, Isograft group showed much better repair, particularly better reorganization of subchondral bones, than allograft group.3) We examined the influence of Fibronectin on the differentiation of cultured chick embryo chondrocytes. As a result, chondrocytes changed their shape by adding Fibronectin into the medium, from spherical to spindle form. That is, dedifferentiation of chondrocyte.4) We examined immunochemically whether the chick chondrocytes have fibronectin receptor on their cell surfaces or not. As a result, we could verify that they recognized RGDS sequence of Fibronectin and had receptors.5) We will make more clear influences of various culture conditions on differentiation and phenotype-maintenance of chondrocytes, for the purpose of repairing the damaged joint surface.