Chromatographic Separation of Proteins Using chitosan Ion Exchangers

使用壳聚糖离子交换剂进行蛋白质的大规模色谱分离在技术上和经济上都是可行的，壳聚糖离子交换剂可以非常便宜地从节肢动物的壳中制备。具体结论如下：1.交联壳聚糖对牛血清白蛋白（BSA）的吸附在含盐溶液中是可行的。当无机电解质共存时，平衡温度受盐浓度和pH值的影响较小。吸附在树脂上的BSA可用pH=9.2的缓冲溶液洗脱。当溶液中除缓冲液外无无机电解质时，强碱交联壳聚糖对BSA的吸附效果较好。平衡等温线受pH的影响很大。pH=7时，吸附量最大。吸附在树脂上的BSA用NaCl水溶液洗脱. BSA的粒内有效扩散系数受BSA浓度和pH值的影响，当BSA浓度低于1 g/dm^3时，吸收数据与表面扩散和孔隙扩散的平行传输机制吻合。当BSA浓度大于1 g/dm^3时，孔扩散为速率控制步骤，收缩核模型与实验数据吻合较好.测定了穿透曲线和洗脱曲线。由于离子交换颗粒是硬的，即使当床很长时，溶液也能很好地流过床。实验穿透曲线与我们提出的分析解很好地一致[Yoshida等人; Chem.Eng.Sci.，39，1489（1984）]。由穿透曲线确定的颗粒内扩散系数与由浅床法确定的颗粒内扩散系数一致。

Large-scale chromatographic separation of proteins using the chitosan ion exchangers, which may be made very cheaply from the shells of arthropods, appears technically and economically feasible. Details of the conclusions are as follows :1. The adsorption of bovin serum albumin (BSA) on the crosslinked chitosan appears technically feasible for salt-containing solutions. When inorganic elecLrolyLes coexisted in the solulioii, Llie equilibrium isol[ierms were liLLIe affected boti by the concentration of the salt and by pil. BSA adsorbed on the resin can be eluted by buffer solution of pH=9.2. The strong base crosslinked chitosan adsorbed BSA well when there were no inorganic electrolytes except for buffer in the solution. The equilibrium isotherms were affected by pH considerably. When PH=7, the amount of adsorption was largest. BSA adsorbed on the resin- was eluted by NaCl aqueous solution.3. The intraparticle effective diffusivity of BSA was affected by the concentration of BSA and pH. When the concentration of BSA is lower than I g/dm^3, the uptake data agreed well with the parallel transport mechanism of surface and pore diffusions. When the concentration of BSA is higher than I g/dm^3, the pore diffusion was the rate controlling step and shrinking core model agreed well with the data.4. Breakthrough curves and elution curves were measured. Since the ion exchange particles are hard, the solution flowed through the bed well even when the bed was long. The experimental breakthrough curves agreed well with the analytic solution presented by us[Yoshida et al ; Chem. Eng. Sci., 39, 1489(1984)]. The intraparticle diffusivities determined from the breakthrough curves agreed with those determined by the shallow bed method.