Immunohistological study of the developing peripheral of chicken with use of a whole-mount staining method

用抗神经丝抗体对13~35期鸡胚进行整体免疫组织化学染色，追踪周围神经及其分支，甚至最细小的管径，并阐明它们与发育中的主动脉弓、心脏和肺的三维关系。在鸡胚的早期，如18期，眼神经比其他两种神经，即上颌神经和下颌神经显示出更显著的形态。免疫组织化学染色显示，三叉神经节位于三叉神经三叉处的轴突网状结构中。异位三叉神经节也发现在这个普通神经节不远，但在表皮层，而不是在表皮下(见Anat和Embryol)。详细观察了发育中的迷走神经的分支及其与发育中的肺、主动脉弓，特别是与发育中的心脏的三维关系。我们已清楚地证明，迷走神经的心脏分支可分为门脉-动脉-心支和门静脉-心支。在23-25期，第4、6弓呈逆时针方向旋转，第4弓位于腹侧，第6弓位于背侧。随着主动脉弓的旋转，左门-动脉-心脏分支位于腹侧，而右门动脉-心脏分支位于背侧。(参见Anat和Embryol)。后者的心脏分支与肺静脉一起进入心脏--通过静脉门。

Chick embryos at stage 13 to 35 were immunohistochemically stained in whole-mount using anti-neurofilament antibody, and the peripheral nerves and their branches, even of finest calibre were traced, and their three-dimentional relationships with the developing aortic arches, the heart and lungs were elucidated.In chick embryos at early stage, e. g. 18 the opthalmic nerve revealed more more remarkable configuration than other two --- i. e., the maxillary and mandibular nerves do. The trigeminal ganglion was immunohistochemically stained at the base of the "tri-furcation" of this nerve, in a reticular network of the neurite. An ectopic trigeminal ganglion was also found not far form this ordinary ganglion, but in the epidermous layer and not under the epidermis (see Anat and Embryol). Branches of the developing vagal nerve and their three-dimentional relationships to the developing lungs, the aortic arch and especially, to the developing heart were thoroughly observed. We have demonstrated clearly that the vagal cardiac branches could be classified into a porta-arteriarum-cardiac branches and a porta-venarum-cardiac branches. The former branches revealed their asymmetric dis-position in accompany with the asymmetric disposition of the aortic arches : At stage 23-25, the 4th and 6th arches rotate in a counter-clock-wise direction, rendering the 4th aortic arch is located ventrally, while the 6th aortic arch located dorsally. Concomitantly with this rotation of the aortic arches, the left porta-arteriarum-cardiac branch become located ventrally, while the right porta-arteriarum-cardiac branch located dorsally. (see Anat and Embryol). The latter cardiac branches found to enter the heart in accompany with the pulmonary veins--via porta venarum.