The Distribution of the Blood Group A- and B-active Glycolipids in the Human Erythrocyte Membrane

对人血A、B和AB型红细胞膜中A和/或B活性糖脂的结构变化分布进行了定量研究。为了检测具有血型特异性的糖脂，使用单克隆抗体在薄层色谱板上进行免疫染色，并使用色谱扫描仪确定每种糖脂的相对抗原比。结果表明，碳水化合物链较短、较简单的血活性糖脂比碳水化合物链较长、较复杂的血活性糖脂在红细胞膜上的分布更大。此外，我们还发现从血液A和AB红细胞膜中提取的4种A-活性糖脂的抗原比率相似，并且从血液B和AB红细胞膜中提取的6种B-活性糖脂的抗原比率也相似。

A quantitative study on the distribution of structural variations of the A- and/or B-active glycolipids in human blood group A, B and AB erythrocyte membranes was performed. In order to detect the glycolipids with a blood group specificity, immunostaining on a thin-layer chromatography plate, using a monoclonal antibody, was applied, and the relative antigenic ratio of each glycolipid was determined by the use of a chromatoscanner.The results indicated that the blood group active glycolipids with shorter and simpler carbohydrate chains were found to have a greater distribution on the erythrocyte membranes than those with longer and more complex carbohydrate chains. Further, it was found that the antigenic ratios of four A-active glycolipids from the blood A and AB erythrocyte membranes were similar, and that this also was observed with regard to the six B-active glycolipids from the blood group B and AB erythrocytes.