Novel endothelial cell culture substrate of polystyrene derivatives

通过在聚苯乙烯表面引入磺酸、季铵盐和羟基等官能团，制备了聚苯乙烯衍生物，并用X射线光电子能谱和动态接触角分析了其表面性质。在有血清和无血清的情况下，内皮细胞在聚苯乙烯表面都有少量的黏附；在有血清的情况下，内皮细胞在磺化聚苯乙烯表面的黏附率很高，与磺酸的含量无关。但在无血清条件下，内皮细胞在磺化聚苯乙烯表面的黏附随着磺酸含量的增加而增加，在含有季铵盐的聚苯乙烯表面也观察到类似的行为，表现为内皮细胞的黏附增殖，其程度与组织培养梯度聚苯乙烯基本相同，这一结果表明内皮细胞的黏附和增殖可以由基质的表面特性来控制。

Culture of human endothelial cells on the surfaces of polystyrene derivatives was examined in terms of cell adhesion cell proliferation and production of prostacycline on these surfaces.Polystyrene derivatives were prepared by introducing functional groups such as sulfonic acid, quartery ammonium salt and hydroxyl-group to polystyrene surface, and their surface properties were analyzed by X-ray photoelectron spectroscopy and dynamic contact angle measurement.Few endothelial cell adhered on polystyrene surface in both case of serum-containing and serum-free condition.In case of serum-containing condition,adhesion of endothelial cells on sulfnated polystyrene surface was high, independent of the content of sulfonic acid. However in case of serum-free condition adhesion of endothelial cells on sulfonated polystyren surface increased with the content of sulfonic acid.The similar behavior was also observed on the polystyrene surface with quartery ammonium salt.These surfaces exhibited the proliferation of adhering endothelial cells, the extent of which was almost the same as tissue culture-graded polystyrene.These results suggest that adhesion and proliferation of endothelial cells could be controlled by surface characteristics of substrates.