Studies on the Valuation of Satsuma Native Fowl as Animal Genetic Resources.

1)以温州蜜柑本地鸡为遗传资源，对2个世代的开产日龄、蛋重、500日龄产蛋量和300日龄体重进行了研究。通过测定排泄物中N ^1-甲基组氨酸的产量，确定了肌肉蛋白质合成和降解的分数率。4周龄时，温州蜜柑本地鸡的合成率和降解率相对较高。3）通过对温州蜜柑本地鸡多态位点比例（Ppoly）、平均期望杂合度（H）和有效等位基因数（Ne）的分析，估计温州蜜柑本地鸡的遗传变异。4）设计了一种用于测定鸡采食量的饲料添加剂，并测定了温州蜜柑土鸡饲料效率的遗传参数。5）从温州蜜柑土鸡等13个品系中提取鸡基因组DNA，用6种限制性内切酶进行酶切，以随机克隆为探针，对酶切后的DNA进行RFLP分析。13个品系的温州蜜柑土鸡杂交带型基本一致。

1) Age at first laying, egg weight, egg production to 500 days of age and body weight at 300 days of age in Satsuma native fowls as animal genetic resources over 2 generations. Heritabilities of the above mentioned traits and genetic and phenotypic correlations among them were estimated.2) Fractional rates of muscle protein synthesis and degradation were determined by measuring the output of N^1-methylhistidine in the excreta in the chicken. The fractional rates of synthesis and degradation at 4 weeks of age in the Satsuma native fowl were relatively high compared with these in the other stocks.3) The proportion of the polymorphic loci (Ppoly), the expected average heterozygosity per individual (H), and the effective number of alleles per locus (Ne) were assayed to estimate the genetic variabilities in Satsuma native fowl. That is, Ppoly=0.22, H=0.08 and Ne=1.1.4) A feeder for measuring chicken feed intake was designed, and the genetic parameters of feed efficiency in Satsuma native fowl.5) Chicken genomic DNAs were prepared from 13 lines of the chicken including Satsuma native fowl and digested with 6 restriction enzymes RFLP was applied to the digested DNA using random clone as a probe. Hybridization banding patterns of Satsuma native fowl were identical among the 13 lines.