An Application of Growth Factor for the Maturation of Premature Infants

肌醇是磷脂酰肌醇的一种成分，磷脂酰肌醇被称为生长因子的第二信使。近年来，肌醇生长因子的作用引起了人们的广泛关注。早产儿生长发育需要多种生长因子，因此需要合理安排生长因子的作用，本研究旨在阐明肌醇在早产儿生长发育中的作用。我们用气相色谱法测定了羊水和新生儿血清中的肌醇。羊水及新生儿血清中肌醇浓度与妊娠时间呈负相关。几个星期了。极低出生体重儿血清中肌醇浓度最高。本研究采用逆转录聚合酶链反应（PCR）技术检测细胞内信使RNA水平，探讨肌醇对极低出生体重儿基因表达的影响。为了证实这种方法，我们分析了杜氏肌营养不良症（DMD）患者的信使RNA。mRNA的逆转录和PCR扩增良好。在一例DMD患者中，外显子19内有一个非常小的缺失，我们分析了信使RNA序列，发现在信使RNA成熟过程中，缺失的外显子被跳过。这一结果不仅说明我们的方法是有效的，而且还说明信使RNA的成熟是由外显子序列控制的，既然我们可以建立信使RNA的分析方法，我们将利用这种方法进一步分析肌醇生长因子的作用机制。

Myo-inositol is a constituent of phosphatidyl-inositol which is known as a second messenger of growth factor. Recently many attentions have been paid for the action of growth factor of myo-inositol. In premature babies who need many growth factors for their growth, the actions of those growth factors should be well organized.Our study is aimed to clarify the growth factor action of myo-inositol in premature baby. We measured myo-inositol in amniotic fluid and serum of the neonates by gas-chromatography. The concentrations of myo-inositol in both amniotic fluid and neonatal serum were reversely correlated with the gestation. al weeks. And very low birth weight neonates showed the highest concentration of Myo-inositol in the serum. These results suggested myo-inositol might have some function for the normal growth of very low birth weight infants.To determine the effect for gene expression of myo-inositol, we employed a reverse-transcriptase polymerase Chain Reaction (PCR) to determine messenger RNA levels in cell. To confirm this method, we analyzed messenger RNA of Duchenne Muscular Dystrophy (DMD) patient. The messenger RNA was well reverse-transcribed and amplified by PCR. In one patient with DMD who has a very small deletion within exon 19, we analyzed messenger RNA sequence and found that the exon with the deletion was skipped during maturation of messenger RNA. This result showed not only our method was useful but also the maturation of messenger RNA is controlled by exon sequence.Since we could establish the method for analysis of messenger RNA, we will further analyze the mechanism of growth factor action of myo-inositol by using this method.