Studies on the Relationship Between Salivary Proline-Rich Protein Polymorphisms and the DNA Loci.

1.纯化了4种慢迁移G1蛋白（Gl 4J、5、6、7），并分析了其氨基酸组成。经鉴定，所有蛋白均为G1蛋白.在与Azen博士的合作研究中，发现了一种新的G1蛋白变体（Gl8），以前对该变体蛋白的研究将其误认为是Pa 2，是PRH 1位点的产物。该个体的另一个PRB 3等位基因为空。在中国人、马来人、印度人和菲律宾人中检查了缓慢迁移的GI变体。这些变异的等位基因仅限于蒙古人种，并且每个等位基因在亚洲人群中都有偏倚。在白人中观察到的Gl 4与我们的缓慢迁移的Gl不同。此外，在菲律宾人中发现了新的等位基因（Gl 10），从而导致PRB 3位点存在12个等位基因.假设基础PRPs由4个位点产生，通过家系研究将PmF、PmS、Ps、G1、Pmo 1、Pmo 2、P3蛋白分为PmF/PmS/Pmo 1、G1、Ps/Pmo 2和P3 4类。PRP基因座具有高度多态性，在法医学和人类学研究中可用于个人身份识别。

1. Four kinds of slow-migrating Gl proteins (Gl4J, 5, 6, 7) were purified and amino acid compositions were analyzed. All the proteins were proved to be the same kinds of Gl protein.2. Under collaborative research with Dr. Azen, new variant of Gl protein (Gl8) was found. A previous study of this variant protein misidentified it as Pa2 and was a products of PRH1 locus. The other PRB3 allele in this individual was a null.3. Slow-migrating Gl variants were examined in Chinese, Malays, Indians and Filipinos. These variant alleles were restricted to Mongoloids, and each allele was biased within Asian populations. Gl4 observed in whites was different from our slow-migrating Gl. In addition, new allele (Gl10) was found in Filipinos, which leads to the existence of 12 alleles in PRB3 locus.4. Assuming that basic PRPs were produced from 4 loci, PmF, PmS, Ps, Gl, Pmo1, Pmo2, P3 proteins were classified through family studies into 4 groups, that is, PmF/PmS/Pmo1, Gl, Ps/Pmo2 and P3.We would like to continue to establish the relationships between salivary basic PRPs and DNA loci. PRP Loci are highly polymorphic and will be useful for personal identification in forensic medicine and anthropological studies.