Study on Clinical Application of Chinese Herbal Medicine as A Biological Response Modifier

本论文对中草药MCX-912进行了沿着临床试验，并结合分析化学、生物化学、细胞生物学、肿瘤学和药理学等方面的研究成果进行了表征。以下是结果总结。(1)MCX-912含有0.8%的K，Fe，Zn，Cu（总量：0.9%），42.0%的蛋白质和7.2%的糖。(2)虽然MCX-912对EMT 6单细胞的细胞杀伤活性很小，但MCX-912显著增强了X照射细胞的致死性损伤。(3)荷SCC VII瘤小鼠单次X线照射后，每天皮下注射MCX-912，放射效应增强1.4倍。使用MCX-912与低氧细胞放射增敏剂和/或热疗可以实现进一步增强。(4)MCX-912促进了芬顿试剂对过氧化氢的分解。(6)MCX-912对S180实体瘤具有抗肿瘤作用。(7)每天向MM 46荷瘤小鼠施用MCX-912不产生抗肿瘤作用。然而，脾细胞的数量增加，从而腹膜内的MM 46细胞越来越失活。(8)对患者施用MCX-912后，淋巴细胞亚群中的细胞毒性T细胞数量增加。(9)MCX-912可改善患者的体力状态，这是由于患者食欲增加，剧烈疼痛减轻或消失。

Clinical assay of Chinese herbal medicine MCX-912, which was extract from earthworm, has been carried out along with characterization based on analytical chemistry, biochemistry, cell biology, oncology, and pharmacology. The followings are the summary of results.(1) MCX-912 contains 0.8% K, Fe, Zn, Cu (total amount : 0.9%), 42.0% proteins, and 7.2% sugars.(2) Although cell killing activity of MCX-912 on EMT6 single cells is small, the lethal damage of X-irradiated cells is considerably enhanced by MCX-912.(3) Administration of MCX-912 everyday to SCC VII tumor bearing mice in their thinghs after single X-irradiation results in 1.4 times enhancement of radiation effect. Further enhancement can be achieved using MCX-912 together with hypoxiccell radiosensitizers and/or hyperthermia.(4) Decomposition of hydrogen peroxide by the Fenton's reagent is accelerated by MCX-912.(6) MCX-912 shows an antitumor effect on S180 solid tumors.(7) Administration of MCX-912 everyday to MM46 tumor bearing mice gives rise to no antitumor effect. However, number of spleen cells increases and thereby intraperitoneal MM46 cells are increasingly inactivated.(8) Number of cytotoxic T-cells in the lymphocyte subsets increases on administration of MCX-912 to patients.(9) Performance status is improved by MCX-912, due to the increase of appetite and the decrease or disappearance of sever pain for patients.