Development of Passive Immunization System Against Dental Caries by Hen Egg Yolk Antibodies of Streptococcus Mutans.

用抗血清型c S的全细胞、无细胞（CF）葡萄糖基转移酶（GTase）、细胞相关（CA）GTase、表面蛋白抗原（PAc）的多克隆卵黄抗体（yIgG），对鸡胚抗血清型c S的效果进行了研究。在体外对S.变形链球菌和保护S.抗龋齿的发展。用福尔马林处理的全细胞、纯化的CF-GTase、CA-GTase或PAc与弗氏完全佐剂一起免疫母鸡（18周龄）。通过硫酸铵沉淀，然后通过DEAE-Sephacel柱层析或用乙醇分级沉淀来纯化yIgG。发现纯化的yIgG是220-kDa的蛋白质，其在加入2-巯基乙醇后解离成H和L链。yIgG对全细胞的免疫反应使S.而CF-和CA-GT酶的yIgG分别特异性抑制这些GT酶的酶活性。抗CA-GT酶和全细胞的yIgG明显抑制S.玻璃表面的变形菌。无特异性病原体的Sprague-Dawley大鼠被严重和反复感染S。变形杆菌和喂食饲料#2000诱导严重龋齿，而喂食含有>0.1%抗CA-GT酶γ IgG的饲料#2000的大鼠导致牙菌斑积累和龋齿发展的统计学显著减少。给予CF-GT酶γ IgG不能防止龋齿。这些结果清楚地表明，抗S.突变体CA-GTase特异性地抑制该生物体的毒力因子，即。e.不溶性葡聚糖合成CA-GT酶，导致龋齿发展的显著下降。

The effect of polyclonal egg yolk antibodies (yIgG) raised against whole cells, cell-free (CF) glucosyltransferase (GTase), cell-associated (CA) GTase, surface protein antigen (PAc) of serotype c S. Mutans was examined in terms of in vitro inhibition of virulence-related factors of S. mutans and protection of S. mutans-infected rats against the development of dental caries. Hens (18 weeks old) were immunized with formalintreated whole cells, purified CF-GTase, CA-GTase or PAc together with Freund complete adjuvant. yIgG was purified by ammonium sulfate precipitation followed by DEAE-Sephacel column chromatography or fractional precipitation with ethanol. Purified yIgG was found to be a 220-kDa protein, which was dissociated into H and L chains upon addition of 2-mercaptoethanol. yIgG to whole cells gave a heavy aggregation of the organisms of S. mutans, while yIgG to CF-and CA-GTase specifically inhibited the enzymatic activity of these GTases, respectively. yIgG to CA-GTase and whole cells was found to suppress clearly the cell adherence of S. mutans to a glass surface. Specific-pathogen-free Sprague-Dawley rats that had been infected heavily and repeatedly with S. Mutans and fed diet #2000 induced severe dental caries, while rats fed diet #2000 containing>0.1% yIgG to CA-GTase resulted in a statistically significant reduction in dental plaque accumulation and caries development. Administration of yIgG to CF-GTase failed to protect against caries. These results clearly suggest that yIgG to S. mutants CA-GTase specifically inhibited a virulence factor of this organism, i. e. Insoluble glucan synthesizing CA-GTase, resulting in a significant drop in the development of dental caries.