Development of Transformation Methods Suitable for Plant Breeding

已报道了许多将基因导入植物的方法。然而，植物育种的最佳方法因情况而异。我们考察了七种转化方法，并试图认识它们的特点。粒子枪不仅被成功地用于观察导入基因的瞬时表达，还被成功地用于构建转基因植物。激光注入法是一种新的转化方法，它显示了标记基因的瞬时表达。嫁接法也在一定程度上观察到了基因转移。用DNA处理的花粉进行杂交，结果表明可用于转化。TI质粒介导的基因转移是一种流行的方法，我们也发现它具有功能性。利用聚乙二醇法直接进行基因转移是构建水稻等转基因单子叶植物的理想方法。不对称细胞融合便于传递大量的遗传信息。

Many methods for introducing genes to plants are reported. However, the best method for plant breeding is different from each case. We examined seven transformation methods, and tried to recognize their characteristics. Particle guns were successfully used not only to observe the transient expression of introduced genes, but also to construct transgenic plants. The Laser injection is a new method for transformation and showed the transient expression of a marker gene. The gene transfer was also observed by the grafting method to some extent. Pollens treated with DNA were used to crossing and showed usable for transformation. Ti plasmid mediated gene transfer is popular, and we also found to be functional. Direct gene transfer by PEG was excellent to construct transgenic monocot plants such as rice. Asymmetric cell fusion was convenient to transfer a large amount of genetic information.