Fundamental Study on the Induction and Prevention of Preleukemia Condition by Bovine Leukemia Virus

从病毒学、免疫病理学和形态学的角度研究了持续性淋巴细胞增多症(PL)的病理特征。实验：供体为一头对BLV抗体呈阳性反应的2岁黄牛，其淋巴细胞数为13,300个/毫升。将3头7~8月龄(A、B、C)犊牛分别静脉接种10^、10.3、10.9、9.6、9.6的淋巴细胞(第2代)。然后，将从牛“A”(BLV抗体阳性，14,868个/nul)中提取的10个淋巴细胞分别静脉接种于另外3头牛(a、b、c)(第3代)。实验仍在继续，用动物A(第四代)的淋巴细胞接种了两只动物。结果：(1)2代动物(A、B)、3代动物(a、b、c)、4代动物(4代)分别于接种后5~8个月诱导出PL。(2)在外周血细胞中，在接种后1-2周，所有动物的T淋巴细胞/B淋巴细胞的比值都出现了逆转。T淋巴细胞(CD4、CD8)数量减少，而免疫球蛋白G阳性B细胞数量增加。(3)光镜下可见染毒24小时的动物外周血淋巴细胞内有大量病毒颗粒。用BLV抗血清进行荧光抗体检测，可在细胞质内检测到BLV抗原。(4)6~9月龄处死的3只PL(A、B、a)动物的淋巴结滤泡增大，构成细胞HD异型性。这些细胞有时对抗肿瘤相关血清(cebl-2)呈阳性反应。

Persistent lymphocytosis (PL) was experimentally prepared as a pre-leukemia condition and its pathological feature was studied from the viewpoint of virology, immunopathology and morphology.Experiment : As a donor, a cattle aged 2 years was used which responded positively to BLV antibody and had PL with 13, 300 lymphocytes/mul. Three calves aged 7-8 months (A, B and C) were intravenously inoculated with lymphocytes of 10^<10.3>, 10^9, 10^<9.6> respectively (second generation). Then, 10^<9.1>, 10^<9.1>, 10^<8.8> lymphocytes taken from the cattle "A"(positive to BLV antibody, 14, 868 lymphocytes/nul) were intravenously inoculated to each of another 3 cattle(a, b and c)(third generation). The experiment has been still being continues by using 2 animals inoculated with lymphocytes from the animal "a" (forth generation). These animals were investigated from the viewpoints of hematology, virology, immunology and histopathology.Results : (1) PL was induced in 2 animals of the second generation (A and B), 3 animals of the third generation (a, b and c), and 2 animals of the fourth generation 5-8 months after inoculation respectively. (2) With periphocytes, at weeks 1-2 after inoculation reversion of the ratio of T lyphocytes to B lymphocytes was recognized in all of the animals. The number of T lymphocytes (CD4, CD8) was decreased, while that of IgG positive B lymphocytes was increased. (3) Electron microscopy showed numerous virus particles in the peripheral lympocytes of the animals with PL which had been incubated for 24hrs. BLV antigens were detected in the cytoplasm when investigated by fluorescence antibody method using antiserum against BLV. (4) It was found that follicles of lymph nodes became larger in the 3 animals with PL (A, B and a) which were sacrificed at 6-9 months and that the constituent cells hd atypia. These cells sometimes responded positively to anti-tumor associated serum (CEBL-2)