Enzyme immunoassay of human Mn-superoxide dismutase and its clinical application : Monitoring for myocardial injury.

本文报道了一种人锰超氧化物歧化酶（Mn-SOD）的酶联免疫吸附试验（ELISA）。MN-SOD分子包括四个相同的亚基，这允许使用相同的单克隆抗体作为捕获和检测器来开发对称测定。该方法提供了一种特异、灵敏、简便的测定人血清中免疫反应性MN-SOD的方法。在最佳条件下，该方法的灵敏度可达2-200 ng。正常人血清锰超氧化物歧化酶（Mn-SOD）水平为99.8 <plus-minus>± 24.8。(mean SD<plus-minus>）和88.8 <plus-minus>20.8（平均<plus-minus>SD）。采用酶联免疫吸附法测定急性心肌梗死患者血清MN-SOD水平。对29例急性心肌梗死患者进行的系列测定表明，血清中MN-SOD呈双相性，在症状发作后约16.2（7.3）h观察到早期小幅升高<plus-minus>，约108（20.6）h观察到高升高<plus-minus>。MN-SOD晚期升高最大值与左室肥厚呈负相关。

An enzyme-linked immunosorbent assay (ELISA) has been developed for human manganese-superoxide dismutase (Mn-SOD), using a specific monoclonal antibody raised against the purified enzyme. The MN-SOD molecule comprises four identical sub-units this permitted the development of a symmetrical assay, using the same monoclonal antibody as both capture and detector. The assay offers a specific, sensitive and convenient means of measuring immunoreactive MN-SOD in human sera. Under optimum conditions, the sensitivily of the assay permits the detection of 2-200 ng of purified MN-SOD from human liver. The mean serum Mn-SOD levels of normal healthy males and females were 99.8 <plus-minus> 24.8. (mean <plus-minus> SD) and 88.8 <plus-minus> 20.8 (mean <plus-minus> SD), respectively. Serum MN-SOD levels were determined in patients with acute myocardial infarction by ELISA. The serial determinations performed on 29 patients with acute myocardial infarction indicated that MN-SOD appears in serum in a biphasic manner ; an early small elevation was observed at approximately 16.2 (<plus-minus> 7.3) h and a high elevation at apporoximately 108 (<plus-minus> 20.6) h after the onset of symptoms. A negative correlation was found between the maximum value of the late elevation of MN-SOD and left ventricul