Preparation and Properties of a Micro Enzyme Electrode for Creatine

以肌酸氨基水解酶（EC 3.5.3.3）和肌氨酸氧化酶（EC 1.5.3.1）为电极，建立了测定肌酸的微酶电极。该传感器由用环氧树脂胶粘剂固定在玻璃管内的铂丝和银丝组成，并通过交联法将肌酸氨基水解酶和肌氨酸氧化酶固定在其尖端。考察了测定肌酸的校准曲线、pH温度、黄素腺嘌呤二核苷酸及伴随化合物的用量对传感器灵敏度和使用寿命的影响。在最佳条件下，传感器在10 μ m ~ 0.65 μ m肌酸浓度范围内呈线性响应。

A micro enzyme electrode using creatine amidinohydrolase (EC 3.5.3.3) and sarcosine oxidase (EC 1.5.3.1) was developed for measuring creatine. The sensor consisted of platinum and silver wires fixed in a glass tubing with epoxy resin adhesive, and creatine amidinohydrolase and sarcosine oxidase immobilized on their tip by cross-linking method. The calibration curve for measurement of creatine, the effects of pH temperature, the amount of flavin adenine dinucleotide and concomitant compound on the sensitivity, and the life-time of the sensor were examined. Under optimal conditions, the response of the sensor was linear in the concentration of 10 muM - 0.65 muM creatine.