Studies on the Seed Production of Undaria Pinnatifida by Tissue Culture Method.

采用组织培养的方法，研究了植物激素对食用褐海带产籽的影响。适合本实验的组织是在拱状叶片周围的中脉处获得的。实验中共使用了11种植物激素，其中5种激素对羽叫形成有效。生长素组分别为3-吲哚乙酸、3-吲哚丁酸和2,4 -二氯苯氧乙酸，细胞素组分别为6-苄氨基嘌呤和Kinetin。生长素组以对氯苯氧乙酸为培养基，细胞分裂素组以6-苄基氨基嘌呤为培养基，观察愈伤组织的种子形成。有效浓度各不相同。前者小于1 ug/l，后者大于100 ug/l。试验结果表明，采用组织培养方法，施用植物激素，可实现羽化和制种；3-吲哚乙酸与对氯苯氧基乙酸、6-苄基氨基嘌呤之间的植物激素组合是有效的。为了明确2,4 -二氯苯氧乙酸在组织培养中形成花萼和种子的有效性，还需要进一步的研究。

The effectivity of plant hormone in the seed production of an edible brown seaweed, Undaria pinnatifida, was investigated by tissue culture method. The suitable tissue for this experiment was obtained from a midrib around arch-like leaves coming out. Eleven plant hormones were used for the experiment, and five of them were effective in calls formation. They were 3-Indoleacetic acid, 3-Indolebutyric acid and2, 4-Dichlorophenoxyacetic acid in auxin group, and 6-Benzylaminopurine and Kinetin in cytokin group. Seed formation-from callus was observed in culture media including p-Chlorophenoxyacetic acid in auxin group and 6-Benzylaminopurine in cytokinin group. The effective concentration was different from each of them. It was showed less than 1 ug/l in former, and more than 100 ug/l in latter.It was suggested from the experiments to make calls and seed production by tissue culture metho applied plant hormones as follows ; the combination of plant hormones between 3-Indoleacetic acid and p-Chlorophenoxy acetic acid, and 6-Benzylaminopurin will be effective. Further investigation should be done in order to clearfy the effectivity of 2, 4-Dichlorophenoxyacetic acid for calls formation and seed formation by tissue culture.