Application of DNA analyses with single locus probes to personal identification and paternity cases

为了在法医实践中提高个人识别和亲子鉴定的准确性，我们检测了七种识别高变DNA基因座的DNA探针。探针和限制性内切酶的组合如下：MR24/1-Hinf L、3‘珠蛋白-PvnII、IIa-ras-PvnII、粘蛋白-PvnII、D2S44(Py N24)1Ysp1、D17S30(PYNZ22)-Ilsp1、DlS57(PYNZ2)-RSA1。多态等位基因分别为37、39、5、10、33、15和5。根据等位基因频率及其在日本人群中的分布，计算排除概率(EP)和亲子指数(PI)。7个DNA探针的累积等电点为0.9999 32，累积等电点为7 3×10 6~770。17种常规血型标志物的累积EP为0.9776，累积PI为1290~0.11。7个DNA探针和CBGM的总EP为0.9999984 78。7个DNA探针的累积等电点是CBGM的4.5-2000倍。因此，单基因座高变数DNA多态被认为是个体识别和亲子鉴定的有用信息。

To improve the accuracy of personal identification and paternity cases in forensic practice, we have examined seven kinds of DNA probes recognizing hypervariable DNA loci. The combinations of probe and restriction enzyme were as follows ; MR24/1-Hinf l, 3 ' Globin-PvnII, Ii a- ras-PvnII, Mucin-PvnII, D2S44(pY NH 24)lYspl, D17S30(PYNZ22)-Ilspl, DlS57(PYNZ2)-Rsal. The polymorphic alleles were 37, 39, 5, 10, 33, 15, and 5, respectively. Exclusion probability(EP)and paternity index(PI)were caluculated from the allelic frequency and its distributions among Japanese population. Cumulative EP from 7 DNA probes was 0.9999 32, and cumulative PI ranged from 7.3 X 10^6 to 770. Also, cumulative EP from 17 kinds of conventional blood group markers(CBGM)was 0.9776, and cumulative PI ranged from 1290 to 0.11. Total EP from 7 DNA probes and CBGM was 0.9999984 78. Cumulative PI from 7 DNA probes were 4.5 to 2, 000, 000 times higher than that from CBGM. Therefore, the single locus hypervariable DNA polymorphisms are considered to be informative for personal identification and paternity test.