Studies on the pathogenesis of cutaneous infections with S.aureus

1)通过免疫组织化学和免疫电子显微镜观察纤维连接蛋白在金黄色葡萄球菌感染中的作用。可溶性纤维连接蛋白在真皮中开始以细小颗粒状沉积，并随着时间的推移逐渐增多。在24 h时，可见两种结合模式。在一种模式中，纤维连接蛋白结合到微生物的细胞表面的一部分。这表明纤维连接蛋白帮助葡萄球菌细胞附着在纤维蛋白或结缔组织上。纤维连接蛋白结合的另一种形式是纤维连接蛋白在细胞周围的结合。这种模式可以解释为纤维粘连蛋白抑制金黄色葡萄球菌附着在纤维蛋白和组织上。2)通过皮肤接种金黄色葡萄球菌建立小鼠实验性葡萄球菌性脓疱病模型，并对病变进行光镜和电子显微镜观察。我们的新方法产生了人类脓疱样水泡，这可能有助于揭示金黄色葡萄球菌在表皮形成水泡的机制。电子显微镜…金黄色葡萄球菌细胞在1/4h和1h侵入角质层，金黄色葡萄球菌细胞与角质细胞之间有明显的晕圈。金黄色葡萄球菌细胞通过纤维状结构附着在周围的角质细胞上。在后来的时间点上没有观察到这些结构。这一过程被认为是建立感染的必要的第一步。3)在评价局部或全身抗菌药物治疗脓疱病的过程中，葡萄球菌脓疱病是通过从人类脓疱病分离的菌株表面接种在小鼠身上产生的。虽然经过充分的化疗后，皮损中的金黄色葡萄球菌细胞形态发生了变化，但存活的金黄色葡萄球菌细胞仍留在角质层中。这可能表明角质层中的微生物不易被抗菌剂根除。4)在小鼠实验性脓毒症模型中，我们观察到金黄色葡萄球菌不能附着在正常皮肤上，但可以很容易地在损伤皮肤和/或巴豆油刺激的皮肤上附着和增殖。较少

1)We observed the role of fibronectin in S.aureus infection using immunohistochemistry and immunoelectron microscopy. Soluble fibronectin began to deposit in a fine granular pattern in the dermis and gradually increased with time. At 24 h two patterns of binding were seen. In one pattern, fibronectin was bound to a part of the cell surface of microorganisms. This suggests that fibronectin helped staphylococcus cells to attach to fibrin or connective tissues. The other pattern of fibronectin binding was the binding of fibronectin all around the cells. The pattern could be interpreted as inhibition by fibronectin of S.aureus attachment to the fibrins and tissues.2)We produced an experimental staphylococcal impetigo model in mice by epicutaneous inoculation with S.aureus and observed the lesions by microscopy and electronmicroscopy. Our new method produce human impetigo-like blister may contribute to disclosing the mechanisms of blister formation in epidermis by S.aureus. Electronmicrosco … More pically, S.aureus cells invaded the horny layer at 1/4 h and 1 h, and a clear halo was seen between S.aureus cells and horny cells. S.aureus cells attached to surrounding horny cells by fibril-like structures. These structures were not observed at later points in time. This process is thought to be a necessary first step for the establishment of the infection.3)Staphylococcal impetigo was produced in mice by epicutaneous inoculation of a strain isolated from human impetigo in the evaluation of topical or systemic antimicrobial agents in the treatment of impetigo. Although after a sufficient chemotherapy S.aureus cells in the lesions morphologically changed, viable S.aureus cells remain in the horny layer. It could suggest that microorganisms in the horny layer are not easily eradicated by antimicrobial agents.4)In the experimental sepsis model using mice, we observed that S.aureus could not attach to the normal skin but could attach easily and proliferated on the injured skin and/or irritated skin with croton oil. Less