Involvement of reactive oxygen species in flap necrosis and development of its prevention by antioxidants.

1)为了证实活性氧的可能参与以及黄嘌呤氧化酶系统在岛状皮瓣缺血再灌注后坏死和带蒂皮瓣远端坏死中的意义，并为皮瓣坏死的修复寻找新的药理措施，我们采用大鼠岛状皮瓣模型和随机模式皮瓣模型进行了一系列实验。结果表明活性氧可能参与岛状皮瓣缺血后坏死和随机皮瓣远端坏死。黄嘌呤氧化酶仅在前者中产生活性氧。2)为了考察外用含有1% SOD的SOD软膏对皮瓣存活率的影响，进行了3次实验。应用于皮瓣下表面或封闭敷料均有良好效果。3)探讨脂质过氧化作用在皮瓣坏死中的作用及外用中药抗氧化剂对皮瓣坏死的修复作用。草本抗氧化剂中，鞣花酸在抑制脂质过氧化水平和提高皮瓣存活率方面的作用最强。4)研究了大鼠岛状脂肪瓣的缺血再灌注损伤。

1)In attempts to substantiate the possible participation of reactive oxygen species, and significance of the xanthine oxidase system in both post-ischemic reperfusion necrosis of the island flap and distal necrosis of the pedicle flap, and to develop new pharmacological measures for salvaging flap necrosis, a series of experiments were made using an island flap model and a random-pattern flap model in rats. The results demonstrated a possible involvement of ROS in both post-ischemic necrosis of island flaps and distal necrosis of random flaps. Xanthine oxidase was significant in producing ROS only in the former.2)In order to examine the effect of topical use of SOD ointment which contains 1% SOD on the enhancement of flap survival, three experiments were made. Its application to the under surface of the flap or occlusive dressing was effective.3)Involvement of lipid peroxidation in flap necrosis and effect of topical application of herbal antioxidant in salvagng flap necrosis were investigated. Among the herbal antioxidants, ellagic acid exerted the strongest effect in suppressing lipid peroxide levels and increasing flap survival.4)Ischemic-reperfusion injury of island fat flap of rats was also investigated.