Electrophysiological and morphological study of endolymphatic sac

（电生理研究）用玻璃微电极测定豚鼠内淋巴囊直流电电位，考察缺氧和各种药物对内淋巴囊直流电电位的影响，了解其性质。结果表明：1)ESP由缺氧敏感部分和缺氧敏感部分组成。2)醛固酮拮抗剂canrenate以剂量依赖性的方式降低ESP。3)乙酰唑胺，一种碳酸酐酶抑制剂，在ESP中产生剂量依赖性的下降，在大剂量时其作用达到饱和。4)儿茶酚胺通过β -肾上腺素能作用可逆地降低ESP。5) ESP的缺氧敏感部分由儿茶酚胺敏感部分和乙酰唑胺敏感部分组成。（形态学研究）观察小鼠内淋巴囊的超微结构及大分子在内淋巴囊中的转运情况。结果表明：1)小鼠ES分为近端、中端和远端三部分。2)中间部分上皮细胞分为I型和II型两种类型。I型细胞具有丰富的微绒毛、胞饮泡、液泡、多泡体、溶酶体和线粒体。II型细胞中这些细胞器的数量较少。3) ES毛细血管分为有孔毛细血管和无孔毛细血管。它们都被认为是大分子可渗透的。ES上皮的基底外侧吸收被认为是活跃的，特别是在中间部分。

(Electrophysiological Study)The endolymphatic sac direct current potential(ESP)was measured in the guinea pig by means of a glass microelectrode, and the effect of anoxia and various agents on the ESP was examined to know its properties. The results are as follows : 1)The ESP is composed of an anoxia-sensitive part and an anoxiainsensitive part. 2)Canrenoate, an aldosterone antagonist, decreased the ESP in a dose-dependent manner. 3)Acetazolamide, a carbonic anhydrase inhibitor, produced a dose-dependent decrease in the ESP with a saturation of its effect at large doses. 4)Catecholamines reversibly decreased the ESP by beta-adrenergic action. 5)The anoxia-sensitive part of the ESP consists of catecholamine-sensitive and acetazolamide-spnsitve parts.(Morphological Study)Ultrastructure of the endolymphatic sac(ES)in the mouse and the transport of macromolecule in the ES were observed. The results are as follows : 1)The ES in the mouse was divided into three parts : proximal, intermediate and distal. 2)The epithelial cells in the intermediate portion were classified into two types : type I and type II. Type I cells had abundant microvilli, pinocytotic vesicles, vacuoles, multivesicular bodies, lysosomes and mitochondria. Type II cells had fewer numbers of these organelles. 3)The capillaries of the ES were classified as either fenestrated or non-fenestrated. They were botn considered to be permeable to macromolecules. The basolateral absorption in the ES epithelium was considered to be active especially in the intermediate portion.