Distribution and function of novel inducing factor for secondary metabolism

1.为了了解IM-2对链霉菌FRI-5的影响，在5 h时加入IM-2，并测量细胞生长、蓝色素产生和抗生素产生。在IM-2的情况下，细胞生长持续到12小时的培养，并没有观察到蓝色色素的生产，直到32小时的培养。与之形成鲜明对比的是，IM-2添加立即终止细胞生长，并且在IM-2添加1小时后观察到蓝色素产生。通过B测定抗生素产量。以枯草芽孢杆菌AT为试验菌株，采用杯法。在IM-2存在和不存在的情况下，透明带的轮廓是不同的，表明产生了不同的抗生素.在IM-2不存在下产生的抗生素在4个纯化步骤后从5升培养液中纯化：产量13.5mg。通过核磁共振、红外光谱和质谱分析，该抗生素被鉴定为D-环丝氨酸，表明链霉菌FRI-5在不存在IM-2.3的情况下可以产生D-环丝氨酸。在IM-2存在下产生的抗生素在6个纯化步骤后从25升培养液中纯化。从该菌中分离得到2个抗生素（C，D）和3个相关化合物（化合物A1，A2和B），化合物C，D，A1，A2和B的产量分别为2.7，3.0，1.3，1.4和2.4 mg。经NMR，IR和MS分析，确定抗生素C和D分别为showdomycin和minimycin。类似地，化合物A1、A2和B是α-秀domycin、α-minimycin和5-α-阿拉伯呋喃糖基-1，3-恶嗪-2，4-二酮。因此，链霉菌FRI-5在IM-2.4的存在下可以产生几种核苷类抗生素和相关化合物。在不存在和存在IM-2的情况下测量这些抗生素的生产概况。在不存在IM-2的情况下，产生D-环丝氨酸，并且没有检测到核苷抗生素，但是IM-2的添加完全抑制了D-环丝氨酸的产生，而是产生核苷抗生素。

1. To know the influence of IM-2 on Streptomyces sp. FRI-5, IM-2 was added at 5-h and cell growth, blue-pigment production and antibiotic production were measured. In the absence of IM-2, cell growth continued until 12-h cultivation, and no production of blue pigment was observed until 32-h cultivation. In clear contrast to it, IM-2 addition terminated cell growth immediately, and blue-pigment production was observed after 1 h of IM-2 addition. Antibiotic production was measured by B. subtilis at as test strain by cup method. In the absence and presence of IM-2, profile of clear zone was different, suggesting the production of different antibiotics.2. An antibiotic produced in the absence of IM-2 was purified from 5-liter of culture broth after 4 purification steps : yield 13.5 mg. By NMR, IR and MS analyses, the antibiotic was identified as D-cycloserine, indicating that Streptomyces sp. FRI-5 can produce D-cycloserine in the absence of IM-2.3. Antibiotics produced in the presence of IM-2 were purified from 25-liter of culture broth after 6 purification steps. Two antibiotics (C, D) and three relating compounds (compound A1, A2 and B) were isolate : yield 2.7, 3.0, 1.3, 1.4 and 2.4 mg for compound C, D, A1, A2 and B, respectively, By NMR, IR and MS analyses, antibiotic C and D were identified as showdomycin and minimycin, respectively. Similarly, compound A1, A2 and B were alpha-showdomycin, alpha-minimycin and 5-alpha-arabinofuranosyl-1, 3-oxazin-2, 4-dione. Therefore, Streptomyces sp. FRI-5 can be concluded to produce several nucleoside antibiotics and relating compounds in the presence of IM-2.4. Production profiles for those antibiotics were measured in the absence and presence of IM-2. In the absence of IM-2, D-cycloserine was produced and no nucleoside antibiotics were detected, but the addition of IM-2 completely suppressed production of D-cycloserine and instead nucleoside antibiotics were produced.