STUDIES ON THE CLEANUP METHOD OF ORGANO CHLORINATED COMPOUND CONTAMINATED SOILS BY MICROORGANISMS

从土壤中分离到一株甲烷利用菌Methylocystis sp. M（菌株M）。这种菌株降解三氯乙烯。菌株M可以降解浓度较高的TCE （35 mg/L）和各种卤代脂肪族化合物。采用70 ml血清瓶和450 ml玻璃柱作为土壤微环境，研究菌株M对tce污染土壤的生物修复作用。将菌株M添加到充满TCE污染的土壤和水混合物的微环境中，TCE浓度为0.1至1ppm。菌株M对0.1 ppm的TCE降解率超过95%，对1,1-、顺式和反式三氯乙烯（DCE）的降解有加速作用。菌株M添加H_2O_2显著促进了TCE的降解。结果表明，菌株M对tce污染土壤具有较好的净化效果。

A methane-utilizing bacterium, Methylocystis sp. M (strain M) was isolated from soil. This strain degrades trichloroethylene(TCE). Strain M can degrade TCE at a relatively high concentration fo 35 mg/L and as well as various halogenated aliphatic compounds. Bioremediation of TCE-contaminated soil by strain M was investigated in soil microcosms, using 70 ml of serum bottles and 450-ml glass columns as the soil microcosms. Strain M was added to the microcosms filled with a TCE-contaminated soil and water mixture with TCE concentration ranging from 0.1 to 1 ppm. More than 95% of TCE of 0.1 ppm was degraded by the addition of strain M, and degradation of 1,1-, cis- and trans-dichloroethylene (DCE) was accelerated by the strain. H_2O_2 addition with strain M significantly promoted TCE degradation. Strain M proved to be very effective to cleaning up the TCE-contaminated soil.