DIRECT ULTRAVIOLET IRRADIATION OF FETAL PANCREAS ZENOGRAFTS FOR REVERSAL OF THE EXPERIMENTAL DIABETIC DOGS

本研究的目的是通过体内直接紫外线照射供体和体外混合胰岛和淋巴细胞培养（MILC）来验证胎胰异种移植物是否能发挥其对糖尿病受体的作用。[材料与方法]将11只全胰腺切除的杂种犬作为经紫外线处理的胎胰移植的受体或对照组，将胎胰组织直接经紫外线照射（紫外线：UVM-57，302 NM，UVP Inc.，辐照时间：10 min）。[结果]紫外线照射可抑制异种移植小鼠胎胰的增殖反应，与未照射组相比，其增殖反应降低至73.4*8.8%（MEAN*SE），存活率较好，全胰切除术后存活天数为84.2*14.1天，另一侧移植物存活天数为84.2*14.1天，与未照射组比较，差异有统计学意义（P < 0.05）。存活时间为24.6*6.5天，超过252.2（] SY.-+。[）66.1天，分别在非移植和同种异体移植中。每日胰岛素需求量、尿糖水平在Zeno移植后呈下降趋势。[结论]紫外线照射后异种胎胰腺免疫反应的改变可能使移植物长期存活，而不需要使用大剂量的免疫抑制剂。

THE PURPOSE OF THIS STUDY IS TO EXAMINE WHETHER FETAL PANCREATIC XENO-GRAFTS CAN EXERT THEIR EFFECT ON THE DIABETIC RECIPIENTS TO USING BY DIRECT ULTRAVIOLET (UV)-IRRADIATION OF THE DONORS IN VIVO AND TO EXAMINE BY THE MIXED ISLETS AND LYMPHOCYTE CULTURE(MILC)IN VITRO. [MATERIALS AND METHODS] ELEVEN TOTAL PANCREATECTOMIED MONGREL DOGS WERE USED TO THE RECIPIENTS OF UV-TREATED FETAL PANCREAS TRANSPLANTATION OR TO THE CONTROL GROUP.THE FETAL PANCREAS TISSUES WERE ENGRAFTED INTO OMENTUM POUCH OR SPLEEN AFTER DIRECT UV-IRRADIATION(UV LIGHT: UVM-57,302NM, UVP INC.,IRRADIATION TIME: 10MIN). [RESULTS] DIRECT UV-IRRADIATION OF FETAL PANCREAS PREVENTED THEM FROM INITIATING PROLIFERATIVE RESPONSES IN XENO-GENEIC MILC.THE RESPONSE REDUCED TO 73.4*8.8%(MEAN*SE)WITH GOOD VIA-BILITY COMPARED WITH NO UV-IRRADIATION.AT THE SURVIVAL DAYS FROM TOTAL PANCREATECTOMY WERE 84.2*14.1 DAYS IN XENOGRAFT.THE OTHER SIDE, THEY WERE SURVIVED AT 24.6*6.5 DAYS AND MORE THAN 252.2(] SY.-+.[)66.1 DAYS,IN NON-TRANSPLANTATION AND ALLOGRAFT RESPECTIVELY.DAILY INSULIN REQUIREMENTS, URINE GLUCOSE LEVELS WERE TRENDED TO DECREASE AFTER ZENO-TRANSPLANTATION. [CONCLUSION] THE ALTERATIONS IN IMMUNE RESPONSIVENESS RESULTING FROM THE USE OF UV-TREATED XENOGENEIC FETAL PANCREAS MAY ALLOW LONG-TERM GRAFT SURVIVAL WITHOUT REQUIRING LARGE DOSES OF IMMUNOSUPPRESSIVE DRUGS.