Architektur der Kernperipherie meiotischer Zellen

使同源染色体在减数分裂中找到彼此的分子机制尚不清楚。间接证据表明，核膜（NE）起着至关重要的作用，因为染色体通过端粒动态地与NE相互作用。更好地了解NE与端粒之间的动态相互作用将为我们对减数分裂机制的认识提供重大进展。最近，我们发现哺乳动物NE的减数分裂特异性结构蛋白（lamin C2）是第一个在减数分裂染色体的附着位点富集的蛋白。在本项目的框架内，我们建议继续对哺乳动物减数分裂细胞核周围的分子结构进行表征。层粘连蛋白C2的作用将在已经存在的敲除小鼠中进行研究，其中层粘连蛋白的基因编码被破坏。此外，将利用酵母双杂交系统鉴定和表征与层合蛋白C2相互作用的蛋白质。第二个减数分裂特异性层蛋白异构体（层蛋白B3）的性质将进一步分析。

The molecular mechanisms that enable homologous chromosomes to find each other during meiosis are not known. Circumstantial evidence indicates that the nuclear envelope (NE) plays an essential role since the chromosomes interact via their telomeres dynamically with the NE. Better understanding of the dynamic interactions between NE and telomeres would provide a significant progress in our knowledge on the mechanisms of meiosis. Recently, we have characterized a meiosis-specific structural protein of the mammalian NE (lamin C2) as the first protein which is enriched at the attachment sites of meiotic chromosomes. Within the frame of the present project we propose to continue with the characterization of the molecular architecture of the nuclear periphery of mammalian meiotic cells. The role of lamin C2 will be investigated in already available knock out mice in which the gene coding for this lamin is disrupted. Furthermore, proteins interacting with lamin C2 will be identified and characterized by using a yeast two-hybrid system. The properties of a second meiosis-specific lamin isoform (lamin B3) will be further analyzed.