Migration and Morphological Change of Wounded Endothelial Cells, and Effects of Flow

1.刮取汇合培养的内皮细胞，在视频显微镜下观察细胞边缘。每个细胞的迁移率为224 ~ <plus-minus>153 μ m/24小时（平均<plus-minus>S.D.）在无钙培养基中孵育24小时后，内皮细胞部分脱落。迁移距离为111 <plus-minus>79 μ m/24小时，小于对照实验，无统计学意义3。ICAM-1和VCAM-1免疫组化染色在伤缘周围的内皮细胞上均为阴性.在胶原凝胶与人体组织共培养系统中，检测了损伤内皮细胞增殖的增强作用。将牛主动脉内皮细胞接种在与人脂肪组织、横纹肌、网膜和静脉混合的胶原凝胶上。人大网膜和横纹肌增强内皮细胞复制.应用内皮细胞和成纤维细胞接种技术建立了生物瓣膜细胞相容性的体外检测系统。

1. Confluent cultured endothelial cells were scraped and the edge were observed by videomicroscope. migration of each cells were 224<plus-minus>153mum/24hours(mean<plus-minus>S.D.) with rndom walkEndothelial cells were detached partially after 24 hours incubation in zero calcium medium. Distance of migration was 111<plus-minus>79mum/24hours and less than control experiment without statically significanc3. Imunohistochemical staining of ICAM-1 and VCAM-1 was negative on endothelial cells around the wound edge.4. Enhacement of wounded endothalial cells reproduction was examined in collagen gel co-culture system with human tissue. Bovine aortic endothelial cells was seeded on collagen gel mixed with human adipose tisuue, striated muscle, omentum, and vein. Human omentum and striated muscle enhanced endothelial cell replication.5. In vitro system to examine cell compatibility of bioprosthesis was developd with applicatin of endothelial and fibroblast seeding.