Proteomic and Transcriptional Profiling of Cartilage

调节软骨形成及其有序过渡到骨的基因表达和信号通路的描述很少。 我们的研究将首次将联合收割机两种互补的尖端方法结合起来，即通过蛋白质组学分析进行蛋白质鉴定和通过微阵列分析进行mRNA分析，以定义这些途径并开发软骨发育和骨形成过程中蛋白质和基因表达模式的全面目录。 这些信息将提供对软骨分化、成熟和结构调节的深入了解，并将为开发更复杂的软骨和骨生物材料以改善组织修复和再生提供关键平台。

Gene expression and signalling pathways that regulate cartilage formation, and its orderly transition to bone, are poorly described. Our studies will, for the first time, combine two complementary cutting-edge approaches, protein identification by proteomic analysis, and mRNA profiling by microarray analysis, to define these pathways and develop a comprehensive catalogue of proteins and gene expression patterns during cartilage development and bone formation. This information will provide insight into the regulation of cartilage differentiation, maturation and structure, and will provide a critical platform for the development of more sophisticated cartilage and bone biomaterials for improved tissue repair and regeneration.