Development of a bead-ELISA kit for rapid identification of enteric bacteria

为了诊断细菌感染，有必要尽早确定致病菌。然而，传统的方法，通过使用培养方法来鉴定细菌需要最短的20-30小时后，获得临床材料。为了评估最近开发的高灵敏度珠-ELISA用于快速鉴定肠道感染的病原菌的工具，我们与印度霍乱和肠道疾病研究所合作，并将珠-ELISA应用于霍乱患者的粪便标本。结果表明，霍乱毒素微球ELISA是一种快速、敏感的检测霍乱毒素的方法，可用于霍乱病人的诊断，并利用空肠弯曲菌的特异性抗原和全菌抗原建立了微球ELISA系统。结果表明，所建立的微球ELISA法可用于空肠弯曲菌的鉴定。因此，我们将尝试研制一种空肠弯曲菌鉴定试剂盒。

To diagnose bacterial infection, it is necessary to identify the causative bacteria as early as possible. However, conventional methods to identify bacteria by using culture methods takes at shortest 20-30 hours after obtaining clinical materials.To evaluate a recently developed highly sensitive bead-ELISA to be used for a tool for the rapid identification of the causative bacteria of enteric infection, we cooperate with Institute of Cholera and Enteric Diseases of India and applied the bead-ELISA for stool specimens of cholera patients. The results showed that the bead-ELISA for cholera toxin is a rapid and sensitive method to detect cholera toxin, thus to diagnose cholera patient.Bead-ELISA system was also developed by using a specific antigen and whole cell antigen to compylobacter jejuni. It was demonstrated that the a developed bead-ELISA is useful to identify Campylobacter jejuni. Thus an attempt will be made to develop a kit for identification of Campylobacter jejuni.