Reconstitution of in vitro testing for metal allergy by means of LPT

为了重建金属过敏的体外试验（LPT:Lymphocyte proliferation Test），进行了以下三个系列的研究。淋巴细胞增殖试验：1)采用Hg^<2+>贴片试验高阳性反应器12个，Co^<2+>贴片试验高阳性反应器5个，阴性反应器17个进行淋巴细胞增殖试验。2) 2-巯基乙醇（2- me）明显抑制Hg^<2+>淋巴细胞的增殖。3)对Hg^<2+>的最佳培养时间为Omol 144hs，对Co^<2+>的最佳培养时间为1.3mu-1.3muM 120hs。4)LPT可能适用于Hg^<2+>和Co^<2+>的敏感性试验，但在临床目的上需要进一步努力才能获得令人满意的可靠性结果，特别是在后者方面。2 .金属致敏小鼠的诱导：通过皮下或腹腔注射变应原联合表皮细胞成功诱导Hg^<2+>致敏小鼠（Balb/c）。表皮细胞变应原结合组分：5% Hgcl^2作用于小鼠尾部皮肤表面24h后，与表皮细胞微粒体、线粒体和溶酶体结合的Hg^<2+>分别为40%、33%和23%。

In order to reconstitution of in vitro testing (LPT:Lymphocyte Prolifiration Test) for metal allergy, following three series of investigation were made.1. Lymphocyte Prolifiration test: 1) Twelve highly positive reactors to patch test with Hg^<2+>, 5 highly positive reactors to patch test with Co^<2+> and 17 negative reactors to those, were participated in Lymphocyte Proliferation test(LPT). 2) A lymphocyte proliferation by Hg^<2+> was markedly depressed with 2-mercaptoethanol (2-ME) in the culture medium. 3) It was suggested that optimal incubation of metal sensitized lymphocyte was 144hs with Omol for Hg^<2+> and 120hs with 1.3mu-1.3muM for Co^<2+>.4) It was concluded that LPT might be applicable for sensitivity test to both Hg^<2+> and Co^<2+>, although further efforts should certainly be need to get results with gratified reliability in clinical purpose, especially in the former.2. Inducement of metal sensitized mice: Hg^<2+> sensitized mice(Balb/c) were successfully induced by subcutaneous or intraperitoneal injection with allergen-conbinant-epidermal cells.3. allergen-bind-fractions of the epidermal cell: After 24hs of application of 5% Hgcl^2 on skin surface of the mouse tail, amounts of Hg^<2+> bound with the microsome, with the mitochondria and with the lysosome of the epidermal cells were regarded as 40%, 33% and 23%, respectively.