Development of a system for rapid protein structure

1)利用两种采用“成像板”的二维x射线衍射仪开发了快速蛋白质结构解析系统a)实验室型衍射仪基于商业产品，RIGAKU R-AXIS IIc和b) Sakabe教授开发的Weissenberg相机)安装在同步辐射源上。(2)利用以太网数据传输联动，建立了A)上述两种衍射仪系统采集的x射线衍射数据与b)本实验室已建立的计算机图形系统之间的接口系统。3)利用上述系统1)和2)，已经建立了以下蛋白质的晶体结构（以从头开始的方式）或在高分辨率下精炼：干扰素- β， RNase Rh， ssi -胰蛋白酶复合物，突变ssi -枯草菌素复合物和野生ssi -枯草菌素复合物。

1) system for rapid protein structure elucidation has been development making use of the two kinds of two-dimensional X-ray diffractometer employing "imaging plates" a) Laboratory-type diffractometer based on a commercial product, RIGAKU R-AXIS IIc and b) Weissenberg camera developed by Prof. Sakabe) installed at the synchrotron radiation source, Tsukuba.2) A system of the interface between a) X-ray diffraction data collected by the above two diffractometer systems and b) the computer graphic system already established in the present laboratory, has been established making use of the Ethernet data transfer linkage.3) Making use of the systems 1) and 2) described above, crystal structures for the following proteins have been either established (in a de novo fashion) or refined at a high resolution : Interferon-beta, RNase Rh, SSI-trypsin complex, Mutant SSI-subtilisin complex and Wild SSI-subtilisin complex.