Development of Crosslinkage-free Linear Polymer Electrophoresis

毛细管电泳用内径为50至100微米的窄熔硅管进行。毛细管中的液体几乎不受地球上其他地方不可避免的对流的影响。在那里，聚合物溶液可以作为凝胶的替代品，广泛用作蛋白质和核酸等生物聚合物的分子筛。这种可能性已为人所知，但并未导致实际应用。本项目从倡导该方法与毛细管电泳联用可能具有实用价值开始。大多数毛细管电泳界的研究人员只对该技术的应用感兴趣，只是为了分析。在本项目中，我们通过研究介质的筛选机理，努力开发以聚合物溶液为分子筛的毛细管电泳法更复杂的应用。已经进行了以下研究：1)考察了使用聚合物溶液时伴随的技术问题，以建立一个陈化程序；2)通过使用一系列具有极窄分子量分布的多糖普鲁兰，明确了聚合物尺寸和分离效率之间的相关性；3)使用葡聚糖进行了类似的研究，以获得在十二烷基硫酸钠存在下分离蛋白质的相同知识。研究结果表明，通过调节聚合物的粒径和浓度，可以有效地控制分离效率。

Capillary electrophoresis is carried out using a narrow fused silica tube with an internal diameter of 50 to 100 micrometers. Liquid in the capillary is virtually free from convection elsewhere inevitable on the earth. A polymer solution can be used there as a substitute for a gel widely used as the molecular sieve for biopolymers such as proteins and nucleic acids. The posibility has been known without leading to no practical application. The present project started by advocating that the approach might be of practical value if combined with capillary electrophoresis. Most researchers in the capillary electrophoresis field just interested in application of the technique just for analysis. In the present project to make it unique, we made effort to develop more sophisticated application of the capillary electrophoresis using a polymer solution as molecular sieve through investigation of the mechanism of sieving of the medium. Following studies have been made : 1) technical problems accompanied by the use of a polymer solution were examined to establish a rutinized procedure ; 2) correlation between polymer size and separation efficiency was made clear by the use of a series of polysaccharide, pullulan, with extreme narrow molecular weight distributions ; 3) a similar study was carried out using dextran to obtain same knowledge about separation of proteins in the presence of sodium dodecyl sulfate. Obtained results made clear that the separation efficiency in the technique can be controlled efficiently through adjustment of the polymer size and its concentration