DEVELOPMENT OF BIOREACTOR SYSTEM WITH PRODUCT SEPARATION

本研究的目的是开发反应萃取法或液膜法从发酵液中原位回收生物制品的工艺。首先，考察了各种生物产品与萃取剂的萃取平衡，以获得反应萃取的基础知识。在此基础上，利用液体表面活性剂膜(以下简称LSM)对多种氨基酸、多肽、有机酸和抗生素进行了分离和浓缩研究。最后，建立了乳酸的原位萃取发酵体系。1.氨基酸和多肽的分离以含三正辛基甲基氯化铵(TOMAC)的LSM为载体，进行了氨基酸和多肽的分离和浓缩。为了阐明萃取机理，对萃取剂的萃取平衡和动力学进行了研究。萃取平衡常数与…有很好的相关性氨基酸和多肽的疏水性更强。2.抗生素的分离(1)研究了不同萃取剂和缓冲液对头孢菌素C的反应萃取和反萃过程。在TOMAc和碳酸盐缓冲溶液中，反应萃取效果较好。这是第一例头孢菌素的提取。(2)采用LSM法提取青霉素G。对LSM法提取青霉素G进行了研究，建立了从发酵液中提取青霉素G的新工艺。以Eca 4360J为油相，乙酸正丁酯和煤油的混合液为乳状液，采用LSM快速分离富集青霉素G。有机酸的分离通过采用多种萃取剂和稀释剂的组合，对发酵液中乳酸的反应萃取进行了研究，以开发一种新的回收技术。结果表明，用溶剂化试剂作萃取剂，用氨水调节发酵液的pH值至5即可实现原位萃取。以正己烷为萃取剂，成功地进行了萃取发酵。较少

The purpose of the present research is to develop the in situ recovery process of bioproducts from the fermented broth by the reactive extraction or the liquid membrane techniques. First, the extraction equilibria of various bioproducts with extractants were examined to obtain the fundamental knowledge of reactive extraction. On the basis of these results, the separation and enrichment of various amino acids, peptides, organic acids and antibiotics were studied by employing liquid surfactant membranes (henceforth LSM). Finally, in situ extractive fermentation system was established for lactic acid. The following results were obtained.1.Separation of amino acids and peptidesThe separation and enrichment of amino acids and peptides were carried out with LSM containing tri-n-octylmethylammonium chloride(TOMAC) as a carrier. To clarify the extraction mechanism, the extraction equilibrium and kinetics were investigated. The extraction equilibrium constants could be correlated well with the … More hydrophobicity of amino acids and peptides. The permeation rate of amino acids and peptides through LSM was found to be controlled by mass transfer processes.2.Separation of antibiotics(1) Reactive extraction of cephalosporin CThe reactive extraction and stripping of cephalosporin C were studied by various extractants and buffer solutions. The reactive extraction occurred satisfactorily when TOMAC and carbonate buffer were employed. This was the first example of cephalosporin extraction.(2) Extraction of penicillin G with LSMThe extraction of penicillin G by LSM was examined to develop a new recovery process from the fermented broth. Penicillin G could be rapidly separated and enriched by LSM where the oil phase of the emulsions was composed of ECA 4360J and the mixture of n-butyl acetate and kerosene.3. Separation of organic acidsThe reactive extraction of lactic acid from the fermented broth was examined to develop a new recovery technique by employing various combinations of extractants and diluents. It was concluded that in situ extraction was possible by using solvating reagents as extractants and adjusting the fermentation broth to pH 5 by ammonia. The extractive fermentation with TOPO-hexane solution proceeded successfully. Less